Hi Kavya,
More than one crystal?
Epitaxial growth (protein crystal growing on salt crystal or vice versa)
Did you try IZIT?
(https://hamptonresearch.com/product-Izit-Crystal-Dye-33.html).
I have seen salt and protein crystals in the same drop before now - but not
growing together.
Ben
On 3 Feb 2023, at 22:15, CRAIG A BINGMAN
<000021371e2fba31-dmarc-requ...@jiscmail.ac.uk> wrote:
Oxidation products of TCEP crystallize in the presence of zinc ions. These
crystals fluoresce strongly under UV, which seems like a dirty trick, if your
expectation is that only protein crystals can do that. The brightfield white
light image of the crystals is also consistent with not-protein crystals,
because of the high refractive index contrast between the crystals and the
mother liquor.
From: CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK
<mailto:CCP4BB@JISCMAIL.AC.UK>> on behalf of kavyashreem
<kavyashr...@instem.res.in <mailto:kavyashr...@instem.res.in>>
Date: Friday, February 3, 2023 at 2:32 AM
To: CCP4BB@JISCMAIL.AC.UK <mailto:CCP4BB@JISCMAIL.AC.UK> <CCP4BB@JISCMAIL.AC.UK
<mailto:CCP4BB@JISCMAIL.AC.UK>>
Subject: [ccp4bb] Regarding the diffraction image
Dear all,
We crystallized a protein (30kDa) + ligand (by cocrystallization), in the
condition 10%PEG3350, 50mM Zinc acetate.
Protein was in the buffer 20mM HEPES, 150mM NaCl, 1uM ZnCl2, 4mM TCEP, pH 8.
Crystal: Crystal: crystal
under UV m
<b06fc576.png> <e091c7fd.png> <8ef9453e.png>
When we collected the data at an in-house facility, it looked something like
this:
<b903961d.png>
The minimum resolution spot is around 9Ang and maximum ~2.2Ang.
I have not come across a protein diffraction like this, nor of a salt. When I
ran the gel for the incubated protein (protein+ligand), there was no
degradation.
Although, I was sure there is some problem with this image I tried processing,
which could not be, But indexing showed a unit cell of 11Ang, 11Ang, 46Ang in
P3. which was quite expected for two of the axes but not the third.
Can anyone please shed some light on this diffraction image?
How can it happen?
Thank you
Regards
Kavya
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