Dear CCP4BB,
I am working on a structure refinement and came across a blob of density
that looks like an anvil (see link). Has anyone seen this before and have
an idea of what it is?
https://drive.google.com/file/d/1BGNwV3MujYjrV4W5m1bh3_WWd49RhJ1w/view?usp=sharing
The protein is in potassium p
king at your pictures, as far as is possible without the ability to
>> rotate them, I would try to fit deoxyribose-phosphate, or even a complete
>> nucleotide. Maybe your prep contained some unreacted nucleotides and your
>> anomalous peak is phosphorus.
>>
>>
>>
rgy? Would you expect
> significant anomalous diffraction from As at this wavelength?
>
> On Aug 21, 2017, at 11:37 AM, Betty Chu wrote:
>
> Hi Shailesh,
>
> When I modelled in the Barium ion with octahedrally coordinated waters and
> ran the refinement, the distances fro
Kumar Tripathi,
> Phone: 9686289668
>
>
> On Mon, Aug 21, 2017 at 9:17 PM, Betty Chu wrote:
>
>> Yes, I have. The cacodylate ion does not fit well into the density.
>>
>> On Mon, Aug 21, 2017 at 11:44 AM, Pradeep Pallan > > wrote:
>>
>>> Did you
Yes, I have. The cacodylate ion does not fit well into the density.
On Mon, Aug 21, 2017 at 11:44 AM, Pradeep Pallan
wrote:
> Did you try modelling in a cacodylate ion (CH3)2AsO2-?
>
> On Mon, Aug 21, 2017 at 10:19 AM, Betty Chu wrote:
>
>> Dear ccp4bb,
>>
>> I am
BaCl2, and 30 mM NaCaC pH 6.4. I have tried modelling
Ba2+ with coordinated waters, MPD, and sodium cacodylate into the electron
density, but none of those fit well.
Any suggestions regarding the identity of this electron density is much
appreciated. Thank you!
Sincerely,
Betty Chu
Paukstelis
