Dear All,
In the spirit of complementary methods (e.g. see Acta Cryst.D Vol 71), I would
like to bring to your attention an upcoming bootcamp on“Isothermal Titration
Calorimetry Analysis”, to be held 10-11 September, 2015at the NIH in Bethesda,
MD, USA. Topics will include basic principlesan
I once encountered mold-dependent crystallization of a protein. Wouldn't that
have made for a lively Methods section?
Luckily, we determined the structure from crystals derived from a different,
non-moldy condition. Whew.
Chad
From: Artem Evdokimov
To: CC
Tom,
How about Magalhaes et al., J. Protein Chem., Vol. 13, p 195?
Chad
From: Tom Peat
To: CCP4BB@JISCMAIL.AC.UK
Sent: Thursday, March 27, 2014 8:09 PM
Subject: Re: [ccp4bb] question on charge charge interactions
Hello Joel,
I like the example of HI
Dear All,
I am very pleased to announce that a workshop entitled
“Methods for the Analysis of Thermodynamic and Hydrodynamic Properties of
Macromolecules and their Complexes” will be held on May 19-23, 2014 in Dallas,
TX, at The University of Texas Southwestern Medical Center.
I post here because
Dear All,
Apologies for the off-topic post, but I think there might be a number of people
subscribed to this list who also use ITC and would find this useful:
I am pleased to announce the immediate availability of the
software program NITPIC. You may download it from
http://biophysics.swmed.
Dear Kushol & Jerry,
I have to take exception to Kushol's contention about SV. As long as the
buffer
and protein parameters are correct and the sample is well behaved (i.e. not
undergoing dynamic rearrangement on the time scale of the SV experiment, not
aggregated, homogeneous), one can deriv
Posted on behalf of David Chuang, Ph.D.:
Postdoctoral Position in X-ray Crystallography is available in the Department
of
Biochemistry, University of Texas Southwestern Medical Center, Dallas, Texas.
The incumbent will: 1) investigate the structure and function of mitochondrial
protein kinase
Hey, Engin,
I saw this instrument at the Protein Society meeting this summer. Looks
impressive. Be aware that even though the volume will be significantly
smaller, the concentration needed to get a measurable amount of heat will
probably be higher (the Microcal rep I talked to confirmed this t
Hi, Phoebe,
Sorry to jump in late on this one- but I second Stefan's note here. When
soaking dinucleotides (which are poor substrates) into Klenow Fragment xtals, I
noted binding both at the active site and at a crystal interface site that is
likely nonphysiological. The adventitious site is
