[ccp4bb] weak anomalous phasing (magic triangle I3C)

Dear all, I'm working on a 18kD protein, the secondary structure prediction says most of the structure is beta sheets, trying to solve the structure with SAD. Heavy atom soaking gives several datasets with I, W, Au, range from 2.7~3.7A, however, the anomalous signal is pretty weak, I couldn't find

Re: [ccp4bb] How to help crystal grow bigger

>"vary the precipitant/protein ratio" Agree with Enrico. Reducing the concentration of your protein sample to make it form less nuclei, then it might grow bigger. Best R, tiantian On Mon, Sep 5, 2011 at 7:48 PM, Enrico Stura wrote: > dear SnowDeer, > > The first step to see if bigger crystals

Re: [ccp4bb] data processing problem with ice rings

Hi there, Thank you for all your suggestions and generous help, I tried some methods you guys mentioned and learned something new . I really appreciate it. With Kay's help,(after exclusion of the ice rings he found that the data are P1, not P2(1). ) I got my structure solved, there are four copies

[ccp4bb] off topic: PDB data mining

Dear all, I have a question about Protein Data Bank. Does anyone know how to search for structures based on molecular weight. For example, I want to get a list of all the enzymes (molecular weight, say, <10kD), is it possible to manage it? Thank you in advance! -- Tiantian

[ccp4bb] How to set the contour level to a specific value in COOT?

Hi there, I'm making an OMIT map figure with COOT, and I want to set the contour level to a specific value, say, 2.0. Since the initial value is not a whole number, I cannot manage it by scrolling the wheel. Can I change this value by scripting or something like that? Thanks in advance. -- Tiant