Dear all,
I'm working on a 18kD protein, the secondary structure prediction says most
of the structure is beta sheets, trying to solve the structure with SAD.
Heavy atom soaking gives several datasets with I, W, Au, range from
2.7~3.7A, however, the anomalous signal is pretty weak, I couldn't find
>"vary the precipitant/protein ratio"
Agree with Enrico.
Reducing the concentration of your protein sample to make it form less
nuclei, then it might grow bigger.
Best R,
tiantian
On Mon, Sep 5, 2011 at 7:48 PM, Enrico Stura wrote:
> dear SnowDeer,
>
> The first step to see if bigger crystals
Hi there,
Thank you for all your suggestions and generous help, I tried some methods
you guys mentioned and learned something new . I really appreciate it.
With Kay's help,(after exclusion of the ice rings he found that the data are
P1, not P2(1). ) I got my structure solved, there are four copies
Dear all,
I have a question about Protein Data Bank. Does anyone know how to search
for structures based on molecular weight.
For example, I want to get a list of all the enzymes (molecular weight,
say, <10kD), is it possible to manage it?
Thank you in advance!
--
Tiantian
Hi there,
I'm making an OMIT map figure with COOT, and I want to set the contour
level to a specific value, say, 2.0.
Since the initial value is not a whole number, I cannot manage it by
scrolling the wheel. Can I change this value by scripting or something like
that?
Thanks in advance.
--
Tiant