[ccp4bb] WG: Postdoc / group leader position in Mass Spectrometry at University of Bayreuth

2014-08-23 Thread Clemens Steegborn
TripleTOF machine. Applications (including CV with publication list, research experience, PhD certificate, transcripts, and two names and contact information for references) should be sent as PDF or by mail to Prof. Dr. Clemens Steegborn University of Bayreuth Dept. Biochemistry, NW III

[ccp4bb] WG: Murnau conference on Structural Biology

2014-07-08 Thread Clemens Steegborn
rward to meeting you in Murnau! With best regards Clemens --- Prof. Dr. Clemens Steegborn University of Bayreuth Dept. Biochemistry, NW III Universitaetsstr. 30 95447 Bayreuth, Germany phone: ++49 0921

[ccp4bb] Murnau conference on structural biology, Sept 10-13

2014-02-05 Thread Clemens Steegborn
We'd be happy to welcome many of you in September! With best wishes, Clemens Steegborn & Oliver Einsle -- Don't miss the 5th Murnau conference on structural biology - Focus topic: Signal transduction Se

[ccp4bb] AW: [ccp4bb] Calcium ions in enzymes

2013-05-31 Thread Clemens Steegborn
We had a case where Calcium facilitates productive ATP binding (Nat Struct Mol Biol. 12 (2005), 32-7) ... not sure whether one would call that a "catalytic function", but it's certainly not "structural" Best Clemens > -Ursprüngliche Nachricht- > Von: CCP4 bulletin board [mailto:CCP4BB@JIS

[ccp4bb] AW: [ccp4bb] cell disruptor / homogenizer - final summary

2013-04-15 Thread Clemens Steegborn
P.S.: The C5 is what we had in Bochum. Also not perfect, but I guess there’s no perfect machine .. Von: Clemens Steegborn [mailto:clemens.steegb...@ruhr-uni-bochum.de] Gesendet: Donnerstag, 28. März 2013 13:58 An: 'Clemens Steegborn'; 'CCP4BB@JISCMAIL.AC.UK' Betref

[ccp4bb] AW: [ccp4bb] cell disruptor / homogenizer - final summary

2013-03-28 Thread Clemens Steegborn
again to everyone for this helpful feedback! Happy Holidays Clemens Von: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] Im Auftrag von Clemens Steegborn Gesendet: Dienstag, 26. März 2013 19:21 An: CCP4BB@JISCMAIL.AC.UK Betreff: [ccp4bb] AW: [ccp4bb] cell disruptor / homogenizer

[ccp4bb] AW: [ccp4bb] cell disruptor / homogenizer - summary

2013-03-26 Thread Clemens Steegborn
(GEA Niro Soavi), and nitrogen cavitation in a pressure bomb (http://www.ncbi.nlm.nih.gov/pubmed/21041386) were suggested. Thanks for the comments and suggestions. Best Clemens Von: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] Im Auftrag von Clemens Steegborn Gesendet: Monta

[ccp4bb] cell disruptor / homogenizer

2013-03-25 Thread Clemens Steegborn
Dear colleagues, We are currently looking for new cell disruptor/homogenizer equipment, mainly for E.coli work. We currently have a Branson sonifier and a Microfluidics Fluidizer - the latter one keeps causing trouble, and we think about a replacement. We previously also used an Avesti

[ccp4bb] AW: [ccp4bb] Citations in supplementary material

2010-11-22 Thread Clemens Steegborn
Hi Bert, rReferences in Science do NOT contain article titles, like in quite a number of other journals (e.g. JBC, just to mention one of them) . and I agree that it's a pain to find out whether a reference is really of interest to you - but I also agree that it's so much more convenient for the

[ccp4bb] Postdoc position at the University of Bayreuth, Germany

2010-11-17 Thread Clemens Steegborn
Postdoc Position in Protein Crystallography A postdoctoral research position in protein crystallography is available in the group of Clemens Steegborn at the University of Bayreuth, Germany. Research in the laboratory is focused on understanding the molecular signaling mechanisms involved in

[ccp4bb] PhD student position in Structural Biology

2010-01-27 Thread Clemens Steegborn
biochemistry. Additional experience in molecular biology and/or protein crystallization would be an asset. Applications (including CV, research experience, and two names and contact information for references) should be sent to Dr. Clemens Steegborn Ruhr-Universität Bochum MA 2/141 Universitätsstr. 150

[ccp4bb] AW: [ccp4bb] Colored proteins :)

2009-10-21 Thread Clemens Steegborn
Cytochrome c Best CSt > -Ursprüngliche Nachricht- > Von: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] Im Auftrag von > Artem Evdokimov > Gesendet: Wednesday, October 21, 2009 2:25 AM > An: CCP4BB@JISCMAIL.AC.UK > Betreff: [ccp4bb] Colored proteins :) > > Hello CCP4 folks! > > I hav

[ccp4bb] AW: [ccp4bb] Crystallization Reagent

2009-08-11 Thread Clemens Steegborn
Hi Paul, Check out http://www.jenabioscience.com/ Best Clemens > -Ursprüngliche Nachricht- > Von: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] Im Auftrag von > Paul Smith > Gesendet: Tuesday, August 11, 2009 7:46 PM > An: CCP4BB@JISCMAIL.AC.UK > Betreff: [ccp4bb] Crystallization R

[ccp4bb] AW: [ccp4bb] Disulfide bond "survival" in the presence of DTT?

2009-08-11 Thread Clemens Steegborn
Hi Brad, For DosR, a stable disulfide bond was reported even in presence of 150 mM DTT - was reduced only after Thermal denaturation. see J Mol Biol. 2008 Nov 21;383(4):822-36 Best Clemens Von: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] Im Auftrag von Brad Bennett Gesend

[ccp4bb] AW: [ccp4bb] Summary IPTG suppliers (mainly Europe)

2009-07-15 Thread Clemens Steegborn
2240 Campus Drive > Evanston IL 60208 > lab: 847.491.2438 > cel: 773.608.9185 > email: j-kell...@northwestern.edu > *** > > - Original Message - > From: "Clemens Steegborn" > To: "'Jacob Keller'" ; >

[ccp4bb] AW: [ccp4bb] Summary IPTG suppliers (mainly Europe)

2009-07-15 Thread Clemens Steegborn
Hi Jacob, I am not sure I understand why a spread in pricing indicates illegal activity. Wouldn't uniformly high prices indicate something illegal, and very different prices just indicate you have a choice of suppliers, qualities etc?!? And just to mention two more supplier names (again: differen

[ccp4bb] Postdoc position in protein crystallography

2009-05-25 Thread Clemens Steegborn
Postdoc Position in Protein Crystallography A research position in protein crystallography at the postdoc level is available in the group of Clemens Steegborn in Bochum, Germany. Research in the laboratory is focused on understanding the molecular signaling mechanisms involved in aging

[ccp4bb] AW: [ccp4bb] AW: [ccp4bb] Twinned data and maps

2009-03-16 Thread Clemens Steegborn
Dodson Gesendet: Monday, March 16, 2009 12:52 PM An: CCP4BB@JISCMAIL.AC.UK Betreff: Re: [ccp4bb] AW: [ccp4bb] Twinned data and maps But dont all twinned refinement programs output detwinned terms for a map? Certainly REFMAC and SHELX do. Eleanor Clemens Steegborn wrote: > Hi Walter, > > You

[ccp4bb] AW: [ccp4bb] Twinned data and maps

2009-03-16 Thread Clemens Steegborn
Hi Walter, You should definitely detwin data for map calculation if you have a significant twinning fraction (and only for maps; keep using the twinned data set for refinement). We use the CCP4 program detwin. BUT if Shelxl gives bad density, maybe that's simply what you have, a bad density map -

[ccp4bb] AW: [ccp4bb] Broken chain in Pymol display

2009-03-04 Thread Clemens Steegborn
Hi Joe, check chainID and segID; there might be a break in one of them not noticed by phenix because it uses the other one, but then leading to an apparent backbone break in coot because it uses the other one ... Best Clemens -Original Message- From: CCP4 bulletin board [mailto:ccp...

[ccp4bb] Ph.D. and Postdoc positions in Structural Biology

2009-01-23 Thread Clemens Steegborn
Ph.D. and Postdoc positions in Structural Biology Several research positions in Structural Biology are available at Ruhr-University Bochum at the Ph.D. and Postdoc level in the groups of Eckhard Hofmann and Clemens Steegborn. The laboratories are located at Ruhr-University Bochum and offer state

[ccp4bb] suggestions for UV spectrometer

2008-12-04 Thread Clemens Steegborn
Best Clemens --- Jun.-Prof. Dr. Clemens Steegborn Ruhr-University Bochum Physiological Chemistry, MA 2/141 Universitaetsstr. 150 44801 Bochum, Germany phone: ++49 234 32 27041 fax: ++49 234 32 14193

[ccp4bb] Ph.D. Position in Structural Biology / Bochum, Germany

2008-04-15 Thread Clemens Steegborn
, and at least two names and contact information for references) either as pdf files to [EMAIL PROTECTED] or as hardcopy to the address given below. Prof. Dr. Clemens Steegborn Ruhr-University Bochum Dept. Physiol. Chemistry, MA 2/141 Universitaetsstr. 150 44801 Bochum, Germany phone: 0049 234

[ccp4bb] Postdoc position: Structural biology of intracellular signalling systems

2007-08-07 Thread Clemens Steegborn
, research experience, and al least two names and contact information for references) should be sent to Dr. Clemens Steegborn Ruhr-University Bochum MA 2/141 Universitaetsstr. 150 44801 Bochum, Germany Email: [EMAIL PROTECTED] [EMAIL PROTECTED] Prof. Dr. Clemens Steegborn Ruhr

[ccp4bb] Postdoc and Ph.D. student positions

2007-03-22 Thread Clemens Steegborn
contact information for references) should be sent to Dr. Clemens Steegborn Ruhr-University Bochum MA 2/141 Universitaetsstr. 150 44801 Bochum Germany Email: <mailto:[EMAIL PROTECTED]>[EMAIL PROTECTED] <mailto:[EMAIL PROTECTED]>[EMAIL PROTECTED] Jun.-Prof. Dr. Clemens St

Re: [ccp4bb] AKTA prime

2007-02-14 Thread Clemens Steegborn
! Mail Q&A for great tips from Yahoo! Answers users. Jun.-Prof. Dr. Clemens Steegborn Ruhr-University Bochum Dept. Physiol. Chemistry, MA 2/141 Universitaetsstr. 150 44801 Bochum, Germany phone: 0049 234 32 27041 fax: 0049 234 32 14193 email: [EMAIL PROTECTED]