[ccp4bb] Jean-Luc Ferrer

to complete its latest upgrade program, Jean-Luc had been in charge to rebuild the FIP beamline in order to provide the best service to the community, which will become BM07-FIP2. Jean-Luc will be forever missed and remembered. David Cobessi, on the behalf of the whole IBS staff and the RéNaFoBiS

Re: [ccp4bb] linking PLP-Lys

Lys to PLP with covalent bond. I am sure there are many ways do it. My friend would appreciate if you could simplify and explain this so that he could learn it without difficulties. Also I could learn I appreciate your time and help Thanks Rajesh -- David Cobessi Institut de Biologie

Re: [ccp4bb] strict structure based alignment

a pairwise comparison . Is there an other program which does something similar? Best Regards Christian -- David Cobessi Institut de Biologie Structurale 41, Rue Jules Horowitz 38027 Grenoble Cedex-1, France Tel:33(0)438789613 33(0)608164340 Fax:33(0)438785122

Re: [ccp4bb] information received through the AFC: iycr2014

English version: http://www.un.org/ga/search/view_doc.asp?symbol=A/66/L.51 David On 07/04/2012 01:50 PM, Vellieux Frederic wrote: Dear colleagues, Information just received here through the AFC channels: the UN general assembly has declared that 2014 will be the international year of

[ccp4bb] PhD position - Institut de Biologie Structurale (Grenoble)

crystallography will be an advantage. IBS is close to the European large instruments, the ILL http://www.ill.eu/ and the ESRF http://www.esrf.eu/. Please send CV and 2 reference letters to david.cobe...@ibs.fr and jean-luc.fer...@ibs.fr -- David Cobessi Institut de Biologie Structurale 41, Rue Jules Horowitz

Re: [ccp4bb] crystallization of a macromolecular complex

Hi Jan, Have you tried to change the pH? Crystallization at 4°C? David Jan Rash wrote: Dear All, This is about the crystallization of the macromolecular complex which is highly soluble and shows no signs of the aggregation (even at high concentration). We have tried several salts,

Re: [ccp4bb] Regarding BMCD

/tagline_groups_8/*http://in.promos.yahoo.com/groups/ -- David Cobessi Institut de Biologie Structurale 41, Rue Jules Horowitz 38027 Grenoble Cedex-1, France Tel:33(0)438789613 33(0)608164340 Fax:33(0)438785122

Re: [ccp4bb] Refolding of Denatured Protein

. Have you tried several detergents for the purification? Or several buffers with or without salt? David -- David Cobessi Institut de Biologie Structurale 41, Rue Jules Horowitz 38027 Grenoble Cedex-1, France Tel:33(0)438789613 33(0)608164340 Fax:33(0)438785122

Re: [ccp4bb] Refolding of Denatured Protein

)? If it is a transmembrane protein, you should try to use detergents for extraction and during the purification. David -- David Cobessi Institut de Biologie Structurale 41, Rue Jules Horowitz 38027 Grenoble Cedex-1, France Tel:33(0)438789613 33(0)608164340 Fax:33(0)438785122

Re: [ccp4bb] Building structure in COOT

-- David Cobessi Institut de Biologie Structurale 41, Rue Jules Horowitz 38027 Grenoble Cedex-1, France Tel:33(0)438789613 33(0)608164340 Fax:33(0)438785122

Re: [ccp4bb] Transfer Rfree flag

) for example) or CCP4 (CNS---MTZ and use cad). David -- David Cobessi Institut de Biologie Structurale 41, Rue Jules Horowitz 38027 Grenoble Cedex-1, France Tel:33(0)438789613 33(0)608164340 Fax:33(0)438785122