Re: [ccp4bb] SUMMARY - crystallization of proteins with His-tag and/or c-myc tags

I have a more general question that's come up in discussion with former colleagues: what's the largest tag that has been co-crystallized with the target protein? I'm specifically wondering about MBP - we've encountered several proteins that would express decently (and, apparently, correctly folde

[ccp4bb] Radius of Gyration

Hallo, one and all, I was wondering if anyone knew of a quick method (say, a publicly-available script, or subroutine in a CCP4 program) by which one might calculate the Radius of Gyration of a molecule, given its pdb file. Any ideas? Many thanks, Dave Shechner Graduate Student Bartel Laborator

Re: [ccp4bb] Expression vector with NdeI-ClaI sites

ndent Cloning) and "subclone" into any position in a vector. ======= David M. Shechner, Graduate Student Bartel Laboratory Department of Biology Whitehead Institute for Biomedical Research Massachusetts Institute of Technology [EMAIL PROTECTED] ===

Re: [ccp4bb] Crosslinking reagents

ryst. 32. 106-112. Cheers, d.s. ======= David M. Shechner, Graduate Student Bartel Laboratory Department of Biology Whitehead Institute for Biomedical Research Massachusetts Institute of Technology [EMAIL PROTECTED] ===

Re: [ccp4bb] finicky protein

in, or following elution. Cheers, d.s. ======= David M. Shechner, Graduate Student Bartel Laboratory Department of Biology Whitehead Institute for Biomedical Research Massachusetts Institute of Technology [EMAIL PROTECTED] ===

Re: [ccp4bb] Tag, you're in! Flag, V5, etc

At least that's been our experience. Andy (No affiliation with NEB) Hi, everyone: Just to give both sides to this story, though, we've had phenomenally bad luck with the NEB Intein system (tagged on either the C- or N- terminus). The protein expressed and purified beautifully, and post-cleavag

Re: [ccp4bb] 3D Glasses - Vuzix HMD by eDimensional

Well, it's not exactly a solution that could be quickly implemented in the short term, but given the rise of open-source crystallography software, I wonder if modules could be written to let the viewer see 3D objects on a 2D display using "head tracking." You'd have to nod your head slightly ba

Re: [ccp4bb] Codon Optimized Expression

Hi, everyone - long time reader, first time poster: I'm also among the "strongly opposed" camp regarding mixing colonies for expression. Namely, when expressing proteins which might be slightly toxic to their host cells - either as a direct toxin, or by somehow altering the host's gene expressio