Hi François,
Chainsaw should do the job for you if you input a clustal alignment.
Cheers
David
Le 26 sept. 2011 à 17:44, Francois Berenger a écrit :
> Hello,
>
> I have one bound complexe (a ligand + a protein in holo conformation).
> I also have the apo structure for a very similar protein.
>
Hi Jiamu,
to complete the Juergen answer, that's true that the MR-SAD approach is very
powerful and can definitely help in your case. You can use Sharp and add the
information from your partial molecular replacement solution encoded as HL
coefficients and do a MR-SAD phasing.
Here is the procedu
t reference in HKL2000 or CCP4.
> If using CCP4, should I also use imosflm to do integrate?
>
> thanks,
> pengfei
>
> At 2011-05-31 02:32:03，"David Veesler" wrote:
> Hi Pengfei,
> you can combine your files using pointless but it is unclear to me based on
>
Hi Pengfei,
you can combine your files using pointless but it is unclear to me based on
your message if you have scaled independently the two data sets or not.
You should scale these two data sets together using one as reference (doable
using XSCALE or SCALA...).
Cheers
David
Le 30 mai 2011 à 11
Hi Kristof,
depending on what you termed "specific sites" the answer is definitely
yes.
I experienced such a case in the following reference related to the
AcrB structure but I remember that this situation arose also in the
case of the LTC4 structure.
-There is a baby in the bath water: Ac
Dear Dale,
concerning the first part of your request you can use BUSTER and
include your water molecules in ncs restraints.
Here is the link for the wiki explaining that
http://www.globalphasing.com/buster/wiki/index.cgi?AutoBusterExample4chawaterNCS
HTH
All the best
David
Le 15 nov. 10 à 21:4
To measure detergent concentrations in protein samples, the way that I
recommend is ATR-FTIR, it is very accurate, fast (10min ) and
requires as low as 10uL of protein sample.
Here is the reference:
· PVeesler, D. et al. Production and biophysical characterization
of the CorA transport
