Hi Rhys,
I hope you are well. The JOVE article for bicelles is nice, plz check the
video. There is a table in the text section showing some structures that
originated from crystals grown at 4-7 % bicelle conc. using not only the
DMPC/CHAPSO mixture... The other parameter that everyone tries is
Dear CCP4 community,
I am very stunned to hear about the death of Mr. Ashley Bray. He was a friend and colleague when I was in Dublin, where he was doing his PhD in membrane protein crystallography. He knew many
techniques and was dedicated to his work, and to become a good scientist.
Ashley
Hi Frank,
The previous suggestions are great. In my case, I had soft crystals (bend from
180° to ~130-140°) in the loop using the LCP but they still diffracted. There
are a lot of heroic stories on how some people solved a structure, you should
just try as many ways as possible (do not only
Hi,
That is true, Michael.. can't remember the reference, but in some study they found that even if you use a higher MWCO (for example 100 kDa) with DDM (micelle 65-70 kDa), there is still 70-80 % retained detergent in the protein sample.
Raji, another thing I would like to say is that the
Hello Peter,
In addition to the great comments/details, please check the following points I
have now in mind.. since you want to relate the size exclusion peak/profile to
the crystallization:
- occasionnaly, some perfect peaks that you might think are homogeneous
actually correspond to a sample
Hi Theresa,
Here is a comparison between both methods (Table under 6-Summary):http://www.cryst.bbk.ac.uk/pps97/assignments/projects/ambrus/html.htm
If you would like to have an idea about structures of membrane proteins (why was NMR used and what answers they got etc) solved by NMR to
