[ccp4bb] AKTA prime

Dear all, I need to decide between buying an AKTA prime and an AKTA FPLC from GE health care. I understand AKTA prime is a low-pressure system, but because it is too much cheaper than AKTA FPLC, it is still very attractive to me. I will mainly use it for Nickel columns and gel filtration colum

[ccp4bb] Bio-rad DuoFlow

Dear all, Thanks a lot for all the feedbacks on AKTA prime. They are so helpful that I have abandoned the idea of buying one. Now it is a choice between AKTA FPLC and Bio-rad DuoFlow. I heard that DuoFlow is not as robust as AKTA and that its parts break down often. The question is whether qua

[ccp4bb] Fwd: Re: [ccp4bb] AKTA prime

"David R. Cooper" <[EMAIL PROTECTED]> wrote: From: "David R. Cooper" <[EMAIL PROTECTED]> Subject: Re: [ccp4bb] AKTA prime To: Frank Lee <[EMAIL PROTECTED]> Date: Wed, 14 Feb 2007 15:13:18 -0500 Hello, Frank. I seem to be having trouble posting directl

[ccp4bb] more on AKTA and DuoFlow

Dear all, Thanks for the almost 100 replies I have received. I appreciate every one of them very much. Since some asked for a summary, here it is, albeit with more questions. :-) Assuming you buy your own computer and put your equipment in the cold room, (1) AKTA (or whatever it is spelled) p

[ccp4bb] microscope

Hi, I apologize if this has been posted here before, but I will need to buy a standard stereo microscope for viewing crystals. Could anyone give me some suggestions regarding the brand, model, and retailer? Thanks in advance! Frank - Get the

[ccp4bb] One PhD position and one Postdoc position available in structural virology

(1) PhD position One PhD position is available in Dr. Fang Li's lab at the Department of Pharmacology, University of Minnesota Twin Cities. Research involves biochemical and structural studies on proteins that guide invasion and replication of important viral pathogens. Candidates should be

[ccp4bb] One Postdoc Position Available in Structural Virology

Applications are invited for a postdoctoral position in Dr. Fang Li's lab at the Department of Pharmacology, University of Minnesota Twin Cities. Research involves biochemical and structural studies on proteins that guide invasion and replication of important viral pathogens. For more information

[ccp4bb] hydrogen bond between aspartate side chain and main chain carbonyl group?

Hi, In a new structure we observed a hydrogen bond between an aspartate side chain and a main chain carbonyl group. Because both of them are electronegative, I am puzzled. My questions are: does this hydrogen bond make sense at all? any precedent for such a hydrogen bond? I would appreciate

Re: [ccp4bb] hydrogen bond between aspartate side chain and main chain carbonyl group?

Dear all, Thanks for all of your replies. The crystallizatin pH is 5.5, above pKa of aspartate, which is 3.8. In addition, the protein functions at neutral pH, and this aspartate is part of the active site. So it doesn't appear that the aspartate is protonated due to low crystallization pH or

[ccp4bb] different crystal packing at different crystallization temperatures

Dear all, I am looking for examples of different crystal packing at different crystallization temperatures. I would appreciate it if anyone could provide the name of the crystallized protein or, even better, the reference of the crystallization experiment. Thanks, Frank

[ccp4bb] Postdoctoral position available in structural virology

Applications are invited for a postdoctoral position in Dr. Fang Li's lab at the Department of Pharmacology, University of Minnesota Twin Cities. Research involves biochemical and structural studies on proteins that guide invasion and replication of important viral pathogens. Strong background in