[ccp4bb] Heavy-atom derivatives

2015-06-29 Thread Giulliana Rangel
good method? Using platine, Hg, Br... Any suggestions? Thank you so much. Best wishes, -- Giulliana Rangel M.S. Biotechnology Program - UNIFESP Structural Biology Laboratory Phone: +55 (12) 3309-9698 330 Talim St ZIP CODE 12231-280 São José dos Campos - SP/ BR

[ccp4bb] Tev cleavage

2015-05-02 Thread Giulliana Rangel
became the protein more hidrofobic, probably. So, If anyone could help me... Thanks in advance Giulliana Rangel

[ccp4bb] Minimal medium M9 > Semet

2015-02-14 Thread Giulliana Rangel
nimal medium M9 composition but liquid form, there wasn't growing. 2. My e.coli strain is roseta (de3) Thanks in advance Best Regards, Giulliana -- Giulliana Rangel M.S. Biotechnology Program - UNIFESP Structural Biology Laboratory Phone: +55 (12) 3309-9698 330 Talim St ZIP CODE 122

[ccp4bb] The phase problem

2014-11-05 Thread Giulliana Rangel
ed to soak heavy-atom ( Hg, Pt, I, Pb) and the diffraction was good, the results in XDS didnt show so good. I didnt look the heavy atom in density. I appreciate any suggestion and ideas what I could do. Best regards, -- Giulliana Rangel Mestranda PPG-Biotecnologia UNIFESP Laboratório de Bio