Re: [ccp4bb] Problem running BALBES

2009-01-29 Thread Jesse Sundlov
Thank you Fei for your input. Your response inspired me and I found the error I was making (a poorly truncated PDB file). However I have another question regarding BALBES for the brains out there. As I mentioned before, I have a two protein complex with a nice homology model for Protein 1 and

[ccp4bb] Problem running BALBES

2009-01-28 Thread Jesse Sundlov
Hello All, I'm trying to use BALBES locally to perform molecular replacement on data of a two protein complex. Protein 1 is a truncated version of a two domain protein of known structure. Protein 2 has a homologous protein of known structure and ~50% identity. I'm having two related problems -

Re: [ccp4bb] any review on protein-protein complex crystallization?

2008-05-14 Thread Jesse Sundlov
This may be obvious, but I would run a size-exclusion column on the complex first. This will give you an idea as to how well these two proteins stick together. On Wed, May 14, 2008 at 4:34 AM, Maarten Dewilde [EMAIL PROTECTED] wrote: Dear CCP4bb, Can anyone point me to good reviews or