Re: [ccp4bb] small-angle scattering and radiation damage control

2009-07-09 Thread Joerg Stetefeld
we have excellent experiences with cocoamidopropylbetaine (CAPB) especially in SAXS experiments of protein-protein and protein-rna complexes, js At Thu, 9 Jul 2009 17:57:36 -0700, Susan Tsutakawa wrote: Hi Bill, 5-10% glycerol usually helps in the majority of cases. However, some proteins req

[ccp4bb] S-Met replacement

2008-04-16 Thread Joerg Stetefeld
Dear All what are the experiences in the community in terms of physico-chemical property changes if one replace a S-Methionine by a Se-Methionine? Are there structural  a/o functional changes known, e.g. the wild type protein binds a ligand whereas the Se-Met version does not? tha

[ccp4bb] inhouse-SAXS

2007-12-11 Thread Joerg Stetefeld
Hi, we are still in the process to establish a new X-Ray facility and would like to use small-angle X-ray scattering (SAXS) in house. The goal is to study protein-protein complexes of ECM proteins. Can anybody share some experiences on this topic? thank you in advance joerg J