You could try adding detergents. We had a case a few years ago of an enzyme
that was highly active but also highly aggregated. Addition of n-octyl
beta-d-glucopyranoside significantly lowered the degree of aggregation such
that crystals could be grown. Lowering the protein concentration also
help
Two structures of NADH complexes. One is a flavoenzyme. I'm sure there are
many more.
Pejchal R, Sargeant R, Ludwig ML.
Structures of NADH and CH3-H4folate complexes of Escherichia coli
methylenetetrahydrofolate reductase reveal a spartan strategy for a
ping-pong reaction.
Biochemistry. 2005
Can anyone recommend a facility that analyzes the metal content of proteins
by ICP or some other technique?
Thanks.
Jack Tanner
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John J. Tanner
Professor of Chemistry and Biochemistry
University of Missouri-Columbia
125 Chemistry Building
601 S
