[ccp4bb] Large scale insect cell expression

Dear ccp4 community, I'll soon start my own lab and together with other colleagues, would like to establish a facility to do large scale insect cell culture for crystallography. Has anyone had any experience with the wave bag systems for this purpose (for instance from GE)? I have heard that

Re: [ccp4bb] suggestions for cryoprotectant

Dear Friduous, This is also a good paper to look at once you know your crystals diffract at room temperature: https://www.ncbi.nlm.nih.gov/pubmed/10944336 Many of the salts in your condition will be cryo-protective if you increase their concentration to saturated levels. Cheers, Joseph.

[ccp4bb] Post-doc in membrane protein structural biology, Karolinska Institute.

information of at least two references. This should be sent to joseph.br...@ki.se and jesper.haeggst...@ki.se. The deadline for application is the 22nd of June, 2017. Joseph Brock | PhD Division of Physiological Chemistry II Department of Medical Biochemistry and Biophysics Karolinska Institutet

[ccp4bb] Post-doc in membrane protein structural biology, Karolinska Institute.

to joseph.br...@ki.se and jesper.haeggst...@ki.se. The deadline for application is the 28th of April, 2016. Joseph Brock | PhD Division of Physiological Chemistry II Department of Medical Biochemistry and Biophysics Karolinska Institutet Scheeles väg 2 SE-171 77 Stockholm, Sweden

Re: [ccp4bb] Post-doctoral position in membrane structural biology, Karolinska Institute.

Ps - application deadline is the 13th of March, 2017. Best, -Joseph. Joseph Brock | PhD Division of Physiological Chemistry II Department of Medical Biochemistry and Biophysics Karolinska Institutet Scheeles väg 2 SE-171 77 Stockholm, Sweden From: Joseph Brock

Re: [ccp4bb] POINTLESS error: "NormaliseInRuns::apply Unused run 0"

Hi Elanor and bb, I realised this was due to me creating a .HKL file with XDS from Eiger data with a DATA_RANGE that was not a multiple of 50! Apologies for any trouble! All the best, Joseph. Joseph Brock | PhD Division of Physiological Chemistry II Department of Medical Biochemistry

[ccp4bb] POINTLESS error: "NormaliseInRuns::apply Unused run 0"

seInRuns::apply Unused run 0" #CCP4I TERMINATION TIME 21 Jan 2017 00:18:31 #CCP4I MESSAGE Task failed Thanks in advance for the help. Cheers, Joe. Joseph Brock | PhD Division of Physiological Chemistry II Department of Medical Biochemistry and Biophysics Karolinska Institutet Scheeles väg 2

Re: [ccp4bb] structure based superposition

Hi Suda, You can do this in Coot by loading the two structures, then using the tool Calculate- SSM superpose. The rmsd value and sequence identity should be reported within your terminal window. Hope that helps. -Joe Date: Thu, 18 Aug 2011 14:40:32 +0530 From: biobud...@gmail.com

[ccp4bb] Seeding and protease questions

Hi everyone, I have two conditions that I want to try and improve by microseeding. I have been reading the literature on the subject and am a bit confused as to what exactly is the stabilizing mother liquor that is used in the serial dilution of the seed stock. Is this just purified protein