[ccp4bb] Install CCP4 on MacOS Monterey

I am trying to install CCP4 on MacOS 12.7 (Monterey). Unfortunately, the disk image (ccp4 8.0) does not open, despite me following the advice of Ctrl-Click, open ... all I get is the informative window with the instructions how to launch the disk image for MacOS 10.15 and later. Any advice out

[ccp4bb] Postdoc in the group of Aneika Leney at U. Birmingham

Please, see the postdoctoral position advertised below in the broad context of structural biology research using mass spectrometry. For informal inquiries contact my colleague Dr Aneika Leney (a.le...@bham.ac.uk) Klaus === Klaus Fütterer, PhD

Re: [ccp4bb] X-ray crystallography defense questions

Q1: who needs X-ray crystallography - we have cryoEM? Q2: what's the phase problem and is it still a problem (after so many years)? ;-) === Klaus Fütterer, PhD Reader in Structural Biology School of Biosciences LES College

[ccp4bb] Research Associate in Integrated Structural Biology at University of Birmingham

Dear colleagues, Prof Teresa Carlomagno has recently joined the School of Biosciences at the University of Birmingham and is in the process of establishing a new research group in integrated structural biology. Part of that group is the position of a Research Associate position described below

[ccp4bb] TBSGC web site

Does anyone on the ccp4bb know whether the TBSGC site is still accessible somewhere? Klaus === Klaus Fütterer, PhD Reader in Structural Biology (Office hours: Mon 5 - 6 pm, Fri 9.30 - 10.30 am) School of Biosciences LES College

[ccp4bb] Recruitment in Structural Biology/Biophysics

Dear All, The College of Life and Environmental Sciences at the University of Birmingham is seeking to recruit a research group leader in structural biology or a related discipline. Individuals pursuing the study of structure-function relationships in the context of membrane transport or

Re: [ccp4bb] RAxis & fiber diffraction

Sue, Louise Serpell at the University of Sussex, UK specialises on fibre diffraction and has some neat software that may be useful to your cause. You can contact her at [EMAIL PROTECTED] Klaus - Klaus

[ccp4bb] Summary of Crystal Imaging Systems

Summary on Crystal Imaging equipment First, I would like to point out that the replies I received do not amount to a representative survey that would accurately reflect the performance of the instruments mentioned. Not all users of crystal imaging system users read the BB, disappointed user

[ccp4bb] Opinions on Crystal Imaging Systems

Dear CCP4BB community, We are looking into acquiring a crystal imaging system. I'm keen to learn about personal preferences and experiences, perhaps with some technical performance specifics. Points of interest include (but are not limited to) * quality of images (focussing depth, lightin

[ccp4bb] disulfide bond in coot

Does anyone know how to prevent Coot from establishing a disulfide bond between two adjacent Cys residues? I have reason to suspect that the bond is (at least partially) reduced. Thanks. Klaus - Klaus

[ccp4bb] Sequence of crystallised protein fragment

We have a 150 kDa protein that reproducibly crystallises at one of the Hampton Screen conditions. However, we know from SDS gel analysis that the crystals contain only a 45 kDa fragment, that forms through proteolytic cleavage over time. We would like to determine the sequence boundaries of

Re: [ccp4bb] is it Ok to freeze

"... room that was lined with insulated steel walls and that could be flushed with liquid nitrogen." I'm trying to picture this ... did you guys have some kind of LN2- proof SCUBA diving equipment to work in there? Klaus ---

Re: [ccp4bb] discontinuous data wedges in XDS

Is it not sufficient to first integrate the one wedge, then use this wedge as a reference data set when integrating the second one? (REFERENCE_DATA_SET= ../wedge01/XDS_ASCII.HKL) Klaus - Klaus Fütterer,

[ccp4bb] SUMMARY: update of coot via fink

Problem was solved by fink selfupdate fink update-all Thanks to Chris Richardson, who suggested the above, and Andreas Förster, who suggested to be patient when launching updated coot the first time. Klaus -

[ccp4bb] upgrade coot via fink on OS X

I had previously installed coot 0.3.2 using fink on OS X on MacBook Pro (under Tiger). Trying to update coot using fink proved more difficult. Initially I tried sudo apt-get install coot Coot would install but would not start due to a library version mismatch. Then it got messy. I followed in

Re: [ccp4bb] How to make a structure-based multiple sequence alignment on DALI server?

Christoph Gille's program STRAP generates structure-based multiple sequence alignment, which can be combined with sequences for which no structure is available. http://www.charite.de/bioinf/strap/ Klaus -

Re: [ccp4bb] How to calculate the contacts between the dimer-dimer interface

Deep-View (Swiss PDB Viewer) allows to calculate (and visualise) a 'contact surface' between two subunits, by loading the subunits into different layers. Residues contributing to the contact surface can be selected by CTRL- click on the contact surface line in the 'Cavities' menu, available un