Hi Min,
If your proteins have cysteine residues, or you could introduce them
into the sequence, then you could label the interacting proteins with a
fluorescent label, run an SDS-PAGE and quantify the bands with a
fluorescent scanner. See for example Supplementary Figure 1C in Fronzes
et al.
On Sat, 11 Jun 2011, Vellieux Frederic wrote:
If the diffraction pattern originates from 2 crystals (in different
orientations, a case I've had with one large crystal plus a satellite
crystal attached to it in the same loop), it is in principle possible to
crystal. I don't think such a modif
Donghui,
there are several ways to get ORGX ORGY parameters. As Juergen suggested,
you could use the numbers from the header or Mosflm output.
More information on the excellent XDS Wiki:
http://strucbio.biologie.uni-konstanz.de/xdswiki/index.php/Obtaining_ORGX_ORGY
Another option would be to r
Hello,
You could try Disulfide by Design:
http://cptweb.cpt.wayne.edu/DbD/
Good luck,
Konstantin
On Mon, 23 Aug 2010, Jacob Keller wrote:
Dear Crystallographers,
I remember having heard of a program which takes a given oligomeric assembly,
and suggests optimum disulfides to stablize the com
I think the second LLG value is after refinement. But sometimes I see that
the second LLG goes negative and I wonder what it means. For example:
RFZ=3.1 TFZ=17.2 PAK=0 LLG=238 LLG=-603
-Konstantin
On Wed, 30 Sep 2009, Simon Kolstoe wrote:
Dear all,
In the phaser .sol file what do the two LLG
Hi,
the Protein-Protein interface analysis server will calculate Gap Volume
and Gap Volume Index:
http://www.bioinformatics.sussex.ac.uk/protorp/
-Konstantin
--
Konstantin Korotkov, Ph.D.
Research Scientist
University of Washington
Department of Biochemistry
Box 3577
Your case might be different, but it could also be a true Se signal from
Se-Cys incorporated into your protein during Se-Met expression. Depending
on the protocol you used, Se may get incorporated into Cys, especially if
only source of "sulfur" is Se-Met. We have seen such signals from Cys-
con
