Hi Min,
If your proteins have cysteine residues, or you could introduce them
into the sequence, then you could label the interacting proteins with a
fluorescent label, run an SDS-PAGE and quantify the bands with a
fluorescent scanner. See for example Supplementary Figure 1C in Fronzes
et al. (2009) Science 323: 266.
-Konstantin
On Wed, 3 Aug 2011, m zhang wrote:
Dear all,
I have two unrelated questions. Any suggestion on any of them will be
greatly appreciated.
First, we have two proteins that bind each other without a doubt. But
since we have very limited amount of proteins and it takes a long time to
reproduce them, we are very hesitating to try ITC and AUC to find out the
stoichiometry of these complex. So I am wondering if there is any other
way we can try to find the ratio without consuming much proteins?
Second, we are thinking about getting a new incubator for crystallization.
Could anyone here recommend a model or a brand?
Thank you,
Min
--------------------------
Konstantin Korotkov, Ph.D.
Research Scientist
University of Washington
Department of Biochemistry
Box 357742
Seattle, WA 98195-7742
(206)616-4512
k...@u.washington.edu
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