I would warn you that the K3 and K9 chillers have internal hoses which cannot
tollerate water pressure above 125 psi. We had a hose fail from the building
chilled loop pressure. So, be careful adding any pressure with a pump before
the chiller...and maybe consider one after the chiller to
Additionally, once the crystals are ready for the trip, remember to place a
heat sink in with the samples. Planes and tarmacs are seldom at room
temperature.
Kris
Kris F. Tesh, Ph. D.
Department of Biology and Biochemistry
University of Houston
From:
If Paratone oil is too viscous, I recommend perfluoropolyether (PFPE). A lot
of small molecule groups use this because it reduces damage to the crystals.
Kris F. Tesh, Ph. D.
Department of Biology and Biochemistry
University of Houston
From: Kelly Daughtry
If either of the two protein structures has been determined/deposited, I would
check if your unit cell matched one of them.
Kris F. Tesh, Ph. D.
Department of Biology and Biochemistry
University of Houston
From: Tanner, John J. tanne...@missouri.edu
To:
You may want to check that the distance, 2-theta and wavelength are correct.
Kris F. Tesh, Ph. D.
Department of Biology and Biochemistry
University of Houston
From: herman.schreu...@sanofi.com herman.schreu...@sanofi.com
To: CCP4BB@JISCMAIL.AC.UK
Sent: Tue,
I would contact Cole-Parmer (http://www.coleparmer.com/) and see what they
recommend. They have been very helpful in the past.
Kris
Kris F. Tesh, Ph. D.
Department of Biology and Biochemistry
University of Houston
From: Georg Zocher
When using oil on protein crystal mounts I suggest:
1. once the crystal is under oil, remove as much adhered solution from the
crystal surface.
2. if there are any volatile components in your drop condition, presaturate
the
oil with that solvent.
3. consider using perfluoropolyether since
Have you tried adding water to your reservoir and allowing it to vapor diffuse
into the drop?
Kris
Kris F. Tesh, Ph. D.
Department of Biology and Biochemistry
University of Houston
- Original Message
From: Tim Gruene t...@shelx.uni-ac.gwdg.de
To: CCP4BB@JISCMAIL.AC.UK
Sent: Mon,
When I researched this a number of years back, both Taylor-Wharton and
Cole-Parmer techs said nearly all dewar failures were in the epoxy in the neck
of the dewar. This could not be remedied through pumping down the vacuum
jacket as the seal was breached.
They did give some advice to
I know of one bad experience with a common refrigerator as it pertains to the
freezer. The refrigerator was great for the 4 deg crystallization trials, but
the freezer had a frost-free cycle which was destroying protein and
chemicals. The user was keeping lots of their sensitive items in the
Here is a start. It can imply you:
1. did not rotate through the complete unique angle to obtain all reflection
intensities.
2. had the detector too close so did not record reflections which were outside
the detector area. (extending the data resolution beyond the
detector/experimental
I have carried Greiner plates all over the world with no problems, even when
asked what they were. I usually place them in a padded envelope (for
insulation) inside my back pack.
On the other hand, Limbro plates have been a little suspect. I have carried
them in styrofoam boxes, metal lunch
I must agree that the less stressed part of a needle may be the end not
contacting the loop or meniscus, but protrudes out the end/top. One can
carefully manipulate the crystal so that it is not parallel with the pin either
by having a bent loop or setting the crystal slightly diagonal to the
Hey there Chris,
Additionally, I have seen trays set up under Argon atmosphere in glove
bags. They are sealed and left in the bag until inspection where they are
temporarily brought out and returned.
Then, when harvesting, they would use a glass dish (taller than the tray
plus some working
...and I prefer to use PFPE (perfluoropolyether, trade name FOMBLIN) as an
oil since it is less viscous, has no reactivity and is a low temp
lubricant that freezes easily. Small molecule crystallographers have used
it for years.
Kris
-
Kris F. Tesh, Ph D
,
Kris
-
Kris F. Tesh, Ph D
Director, Macromolecular Products
Rigaku Americas Corporation
9009 New Trails Drive
The Woodlands, TX 77381 USA
001 281 362 2300 x 144
- Forwarded by Kris Tesh/Rigaku on 09/01/2009 03:25 PM -
From:
Kris Tesh/Rigaku
To:
CCP4BB
-
Kris F. Tesh, Ph D
Director, Macromolecular Products
Rigaku Americas Corporation
9009 New Trails Drive
The Woodlands, TX 77381 USA
001 281 362 2300 x 144
From:
Kris Tesh kris.t...@rigaku.com
To:
CCP4BB@JISCMAIL.AC.UK
Date:
09/01/2009 04:20 PM
Subject:
[ccp4bb] Fw
A trick that small molecule crystallographers use is to make a packing
diagram where the volume includes both molecules A and B, or maybe even
two of each. In this case, it would display all of C (or maybe even 2 of
C either twisted back on itself (symmetry element near molecule C) or
The obvious difference is that the ratio of drop volume to well volume can
be potentially greater in non-microbatch wells, and can accommodate higher
solvent volume transfers...which is generally in dehydration, but can be
for hydration too.
Other considerations are the slower rate of
Dear Junhua,
Try this simple experiment...put a MiTeGen loop up without crystal or
solution and take am image. (...and then tell us what it looks like)
Kris
Kris F. Tesh, Ph D
Director, Macromolecular Products
Rigaku Americas Corporation
9009 New Trails Drive
The Woodlands, TX
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