, a longer his tag or a strep-tag.
Any thoughts on the comparison between GST and strep and Ni purifications
would be much appreciated. OR are there any other (better) high affinity
purifications, I've missed, excluding expense Ab resins.
Thanks,
Mark Collins
Hey,
I have a GST fusion protein secreted into express5 (with hint of serum),
it does not bind beads. Changing pH with 4xPBS limits ppt and enables
binding but in a scale up I think it makes sense to use a TFF/CFF setup
for concentration and diafiltration.
Does anybody have advice about
Hi
Anybody have suggestions for Mac OsX alternatives?
Thanks in advance,
Mark
Hi
Has anyone tried using video glasses such as MyVu as stereo glasses?
www.myvu.com
Seems like it could be a cheap (~$100/set) alternative to montitors +
other hardware. Resolution maybe an issue.
Just a thought,
Mark
Hi
I also like to try the Hampton additive kit or the easier version; 90%
current condition (at 1.1x) and 10% of any screen you can lay your hands
on (ie hampton 1 2).
Sitting verse hanging drops can change crystal size.
Also in situ limited proteolysis, crazy but it works see for more info
--
Mark Collins
Columbia University
Biochemistry Molecular Biophysics
Black Building 259/201 Office/Lab
212 305 1951 (work)
[EMAIL PROTECTED]
