Hi Phoebe,
We had a case were one ligand was bound in the active site (covalently bound)
and another in a pocket in the same crystal (PNAS, 102, 3599 (2005)).
Regards,
Mathews
-Original Message-
From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On Behalf Of [EMAIL
PROTECTED]
Sent:
Dear Friends,
Sorry for the non CCP4 question. We are planning to purchase a small glove box
to setup crystallization trays under anaerobic conditions. If you have used
glove boxes for crystallization, would you please give me some idea?
We are thinking of getting the 815 series from Plas-labs
Hi Friends,
Here is a summary for my question regarding "Glove boxes for crystallization".
They are given approximately in the order they received. I have also kept the
name and e-mail address for any additional information.
Hope some you will find this information helpful for your research. T
Hi Daniel,
1. You may try Xenon or Kr pressure cell. We have a simple version of these
cells at SSRL (see the link below). Rigaku also has slightly more complex
version. Let me know if you want to try this and you don't have access to a
pressure cell.
http://smb.slac.stanford.edu/public
Hi Jacob,
You could try keeping some inhibitor or other small molecules that could bind
to the protein in the test tube. This may help stabilizing the protein as soon
as it comes out of the column. One of my friends had to use an inhibitor
during the whole purification procedure (luckily this
Dear Jacob,
We have been working on this for an year or so. We have a paper partially in
the review process. Unfortunately, the referees were not very excited about the
idea. However, we are developing some automation to speed up the time consuming
process of placing the drops on the loops.
Th
Dear Pietro,
Thank you very much for sharing your thoughts. I am presuming that you were
thinking about microcapillary feeding to reduce the evaporation of the film on
the loop. Yes, this is a problem esp. with drops with high salt or alcohol. If
there is a way to feed the loop using microcap
Hi Jacob,
Keep regular drops and run a cat whisker through the drops after 4 - 5 hours.
You should have small crystals within 6-10 hrs. If they are still large, use
higher protein concentration or precipitant.
Kind regards
Mathews
-Original Message-
From: CCP4 bulletin board [mailto:
Hi Jacob,
Once I had an issue with introuding CO2 into crystals and I was able to get it
bound by putting a small piece of dry ice into the well solution and keeping
the drop under CO2 pressure using nextal trays with screw cap. Please note in
this case CO2 didn't get bound by using pressure c
One small point:
Just make sure that you are not too off from the contents of the protein
solution. Sometimes protein solution may have a high amount of salt or things
like that and we forget to include atleast half of this concentration into the
cryo solution. This could easily crack the cry
Hi Christine,
Another minor point (mainly for hanging drops) not mentioned in the messages.
When you bring the crystallization tray from lower temperature to room temp.
there is a possibility for condensation and slowly droplets near the protein
drops gets absorbed into the protein drop. If th
Hi Brad,
See below my attempts on this in 2010.
Regards,
Mathews
-Original Message-
From: Mathews, Irimpan I.
Sent: Friday, May 21, 2010 8:23 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: RE: For 24 well Netxal plates (non ccp4 question) ==>> SUMMARY
Dear All,
Thank you for the s
Hi Katherine,
We had a case where we used saturating amounts of NaCl and precipitated the
protein to get rid of a very tight binding ligand (Structure, 11, 677-690,
2003; look at the "preparation of the apoenzyme" section).
Regards,
Mathews
-Original Message-
From: CCP4 bulletin boar
Dear Jobi,
See if you can slowly increase the PEG concentration to 28 - 30% and that will
be a good cryo. Since BIS Tris Propane is the buffer, I think, 28% 3350 should
work fine.
If the crystals crack by going straight to 28% PEG 3350, 0.1M Bis Tris Propane
pH:6.5, 0.2M Potassium thiocyanate
Hi Friends,
Just checking to see whether any of you have experience with Molecular
Dimensions SpectroLight 600 Droplet DLS UV/White light imager or similar
systems.
Thank you very much,
Mathews
I fully agree with Pius.
I am very sorry other CCP users for sending one more message and filling your
mail box for some crazy guys reply.
Thanks,
Mathews
-Original Message-
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Pius
Padayatti
Sent: Monday, October 29,
Dear All,
Qiagen has stopped selling the 24 well Netxal trays and changed to 15 well
trays (almost same price) that doesn't fit with any screens. Do you know of
some source for the 24 well screw cap trays?
Thank you very much for your help,
Mathews
: Tuesday, May 11, 2010 12:44 PM
To: Mathews, Irimpan I.
Subject: RE: [ccp4bb] For 24 well Netxal plates (non ccp4 question)
They were the only source (sigh!)
Maybe they will restart making the original product if enough of us
protests loudly enough (hopes not high).
Thierry
-Original Message
hews
-Original Message-
From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of Mathews,
Irimpan I.
Sent: Tuesday, May 11, 2010 12:41 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] For 24 well Netxal plates (non ccp4 question)
Dear All,
Qiagen has stopped selling the 24 well Ne
Long back we used Prosil solution (I think, 1% solution) for siliconization.
Mathews
-Original Message-
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Goldman,
Adrian
Sent: Thursday, January 31, 2019 8:43 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Is ther
20 matches
Mail list logo