Hi Daniel, 1. You may try Xenon or Kr pressure cell. We have a simple version of these cells at SSRL (see the link below). Rigaku also has slightly more complex version. Let me know if you want to try this and you don't have access to a pressure cell. http://smb.slac.stanford.edu/public/facilities/hardware/pressurecell/ltpc.html 2. You could try NaBr soaks to make Br derivatives for MAD. 3. There are also some Gd small molecules complexes which may do the trick. See Acta Cryst. D59, 118 (2003). kind regards, Mathews
________________________________ From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On Behalf Of Daniel Gutmann Sent: Tuesday, April 17, 2007 10:03 AM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] Hey there, I have crystallised a protein in 30% MPD and it it gives nice native data. However, it seems impossible to get heavy metal derivatives and I read that MPD does chelate heavy metals. Did any of you make similar experiences with xtals in MPD conditions? If so did you find a good solution (introduction of selmet makes the protein ustable) to make heavy metal derivatives? Best Regards Daniel
