I would recommend to look at the GE Superose6 increase 3.2/300 columns if you
aim at high resolution with small sample volumes (you will be very limited in
flow rate though).
For a much faster analysis with sufficient resolution, try the 5/150GL columns.
Attached to an HPLC you can have runtime
Hi Jan!
Phenix can do that - have a look here:
http://www.phenix-online.org/documentation/superpose_maps.htm
Best,
Matthias
Date: Thu, 29 Aug 2013 17:17:36 +0200
From: lohoefener@mh-hannover.de
Subject: [ccp4bb] Superposition of electron density
To: CCP4BB@JISCMAIL.AC.UK
Dear all,
Hi all,
concerning the staining, we get good results with a self-made "instant blue".
First staining is achieved within 30min, full staining after maximal 4 hours I
would say. You don't need to destain (still possible with H2O) since the
background is not stained. Sensitivity is better than st
The Berger laboratory at the European Molecular Biology Laboratory (EMBL)
Grenoble, France seeks to recruit an outstanding postdoctoral scientist in
structural biology with a research focus directed towards the structure of
macromolecular assemblies. The major theme within the group is the stru
Another quite powerful tool is ESPript (
http://espript.ibcp.fr/ESPript/ESPript/ ), which takes e.g. clustalw outputs
and allows various manipulations (change of colors, add text, secondary
structures, etc.)
Matthias
From: CCP4 bulletin board [CCP4BB@JI
The Schaffitzel laboratory at the European Molecular Biology Laboratory (EMBL)
Grenoble, France seeks to recruit outstanding postdoctoral scientists in
structural biology. We study the structure and function of large macromolecular
complexes in gene expression. The project involves structure de
Hi James,
You can do this with MOLEMAN / MOLEMAN2 quite easily (see the manual here:
http://xray.bmc.uu.se/usf/moleman2_man.html)
Best
Matthias
Date: Thu, 10 Mar 2011 14:19:21 +
From: james.pearce.h...@gmail.com
Subject: [ccp4bb] Reporting average B values for solvent, chain, ligand etc
Hi Mark,
just use the "Windows Movie Maker" - it's easy to use and comes along with
Windows (or can be downloaded via microsoft.com). This will help you to
generate .wmv output files of any kind of video and / or picture files you
provide as input files. The best possible quality output format
Dear John,
there is also a special mailing list for CCP4MG issues - however, it seems not
to be used frequently... Just wanted to mention that there is one.
Regarding your problems:
I'm using CCP4MG (2.4.1) on several Windows7 (64 bit Prof.) machines and
everything works just fine; with the exc
Dear Jane,
try to split your protein into two chains (e.g. domain 1 --> chain A / domain 2
--> chain B in a PDB file) and use the PISA server for processing. Besides
several interfaces with symmetry mates, one should represent your domain/domain
interface based on chain A/B you've chosen.
Gr
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