If your SPR data is correct, you'll need to use a micro molar protein
concentration (or higher due to dilution in the column) in your analytical size
exclusion chromatography assay. What concentration did you use? Also, ideally
the buffers in both assays should match. And maybe your SPR data is
I'm really sad to hear that. Rest in peace.
One of his articles, which has William as the first author, is very
interesting, well-written, easy to read, and I use it to illustrate and open
the mind of biochemistry students regarding several important topics. Works
like a charm.
RNA
Dear Jacob,
You did not miss the 101 class, it is just too much to remember.
E. coli metabolome when growing on glucose (Lehninger, 2013):
http://www.fullonline.org/science/ecoli_metabolome.png
The book does not provide explanation for this, but I remember reading
somewhere else that
rator..
> ctruncate tests each possible twin operator and scores them. (And so
> does XTRIAGE of course..)
> On 30 November 2016 at 14:00, Keller, Jacob <
> kell...@janelia.hhmi.org > wrote:
> > What about spacegroups in PG 32, e.g., p3212?
>
> > JPK
>
>
Dear CCP4ers,
I'd like to kindly ask your advice. Sorry for the long e-mail.
I have crystals of a 12.3 KDa protein that grow in hexagon-like patterns, link
for the crystal image:
http://fullonline.org/science/cryst01.jpg
XDS, Phenix and Pointless always suggest that the data sets for these
Hi Grant,
I think pymol's module AngleBetweenHelices might be worth checking, at least
for some ideas in the code.
http://www.pymolwiki.org/index.php/AngleBetweenHelices
It has four methods to fit the helix, and a couple of them work well with
b-strands.
Have fun.
Regards,
Napo
-
