Re: [ccp4bb] the structures of Nucleic acid

Dear Fu Xingke, Depends on what Nucleic Acid you are talking of. If it is RNA, you can expect some sequence to tertiary structure correspondence so you might be able to try more MR as compared to DNA. DNA may have double helical architecture but less sequence to tertiary structure corr

Re: [ccp4bb] Structure prediction - waiting to happen

I absolutely agree with Guenter. This non experts speaking wrong things is increasingly happening nowadays. Thanks Guenter, for pointing this out. A theory unless it is given experimental data derived evidence it is still a prediction (just like weather prediction, unless it is realised in the p

[ccp4bb] CEM3DIP Society of India, 3rd Annual Symposium on Single particle CryoEM and Cellular Tomography, 17th Dec 2022

Dear all, *First Announcement.* *3rd Annual Symposium on Single particle CryoEM and Cellular Tomography, 17th Dec 2022 at IISER Pune (Hybrid Mode)* Cryo Electron Microscopy and 3 Dimensional Image Processing (CEM3DIP) Society of India ( http://cem3dipsi.iisertvm.ac.in/), in association

Re: [ccp4bb] Checking X-ray sequence (no more protein).

Dear Jon, We did exactly the same back in 1999's. 1) Natesh R, Bhanumoorthy P, Vithayathil PJ, Sekar K, Ramakumar S, Viswamitra MA. (1999). Crystal structure at 1.8 Å resolution and proposed amino acid sequence of a thermostable xylanase from *Thermoascus aurantiacus*. J. Mol. Biol., 288,

Re: [ccp4bb] sugestions on weak diffracting protein crystals

Dear Deepak, I have a few suggestions: 1) First you can send it to synchrotrons, particularly microfocus beamlines and shoot as many good in-house diffracting crystals. 2) Setting up crystallisation by micro batch method under Al's oil at various vapour exchange rate may help. But

Re: [ccp4bb] request for basic crystallography online classes

Dear Venkata, Check with Birkbeck online crystallography certificate course here: http://www.bbk.ac.uk/study/2020/postgraduate/programmes/TPCCRPRO_C/. Best wishes, Natesh On Wed, 22 Jul 2020 at 09:22, venkata narayana Are < venkat.sai.biochemis...@gmail.com> wrote: > Dear Sir, > > My self Venka

[ccp4bb] Post Doc position : cryoEM

Dear All, A postdoc position is available in my group at the School of Biology (SoB), IISER Thiruvananthapuram (IISER-TVM), Kerala, INDIA. Our campus is arguably one of the most picturesque in India (http://www.iisertvm .ac.in/pages/campus), and is close to the excellent tropical mountain (e

Re: [ccp4bb] Contradictory result between ITC and cocrystal structure

Dear Monika, Did you do the proper positive and -ve control for ITC. It is impossible that there are no changes in thermodynamics.. when it binds.. Either your detector is not sensing it OR your experiment is not running properly.. HTH Best wishes, Natesh On Sat, 22 Feb 2020 at 12:01, monika chand

Re: [ccp4bb] CryoEM course. Third Announcement: EMBO Practical Course CEM3DIP 2020: Single particle cryoEM of macromolecular-assemblies and cellular tomography. Hurry only 6 more day left for registra

* Please write to *cem3dip2...@iisertvm.ac.in * for any queries/sponsorship regarding this course. On Mon, 9 Sep 2019 at 23:16, Natesh Ramanathan wrote: > *Third Announcement: Hurry up. Only 6 more days left for registration !! > **Applicants > from all over the world are encouraged

[ccp4bb] CryoEM course. Third Announcement: EMBO Practical Course CEM3DIP 2020: Single particle cryoEM of macromolecular-assemblies and cellular tomography. Hurry only 6 more day left for registration

*Third Announcement: Hurry up. Only 6 more days left for registration !! **Applicants from all over the world are encouraged to apply.* *EMBO Practical course CEM3DIP 2020: **Single particle cryoEM of macromolecular-assemblies and cellular tomography* *19-30 January, 2020, at IISER & IICB Kol

[ccp4bb] SEC and MALS

Dear Friends, Can you share your experience with examples of MALS giving lower molecular weight (Eg. Monomer) and SEC giving higher molecular weight (Eg. Dimer), for the same protein sample? If you have/know any published paper, can you please point me to that reference paper or

[ccp4bb] EMBO Practical Course CEM3DIP 2020: Single particle cryoEM of macromolecular-assemblies and cellular tomography

*Second Announcement: (Deadline for registration is fast approaching; Travel grants and Child care grants available)* *EMBO Practical course Cryo Electron Microscopy and 3 Dimensional Image Processing (CEM3DIP 2020): **Single particle cryoEM of macromolecular-assemblies and cellular tomography*

[ccp4bb] EMBO Practical Course CEM3DIP 2020: Single particle cryoEM of macromolecular-assemblies and cellular tomography

*First Announcement:* *EMBO Practical course Cryo Electron Microscopy and 3 Dimensional Image Processing (CEM3DIP 2020): **Single particle cryoEM of macromolecular-assemblies and cellular tomography* *19-30 January, 2020, at IISER Kolkata, Kolkata, India.* The *EMBO Practical C

Re: [ccp4bb] 3D

So sad to hear this. Any other alternative hardware for 3D apart from, anaglyph 3D glasses, Freeviewing cross-eyed or parallel 3D viewing methods? Best regards, Natesh On Mon, 11 Mar 2019 at 20:52, David Schuller wrote: > https://www.engadget.com/2019

Re: [ccp4bb] Generating Additional Phasing Statistics

Dear Jason McCoy, You can refine the heavy atom coordinates in mlphare and you should be able to get both the values Phasing Power and Cullis-R for your SIRAS. Best wishes, Natesh On Fri, 1 Feb 2019 at 19:24, McCoy, Jason (mccoyj5) wrote: > Dear CCP4bb members, > > I am compiling a rebu

Re: [ccp4bb] Electron density maps for Cryo-EM structures.

Dear Kevin, Are you referring to "generating a map" from the model (PDB coordinates) generated from tracing the chain in the ab-initio EM map? Best wishes, Natesh On Mon, 10 Sep 2018 at 09:58, Kevin Jin wrote: > Dear All, > > Is there any sever available to create electron density

[ccp4bb] PhD positions in "Structural Molecular Biology" at IISER Thiruvananthapuram.

Applications are invited from highly motivated students for admission to the PhD programme in the Structural Molecular Biology lab of Dr. Ramanathan Natesh in the area of Infectious Disease (*Mycobacterium tuberculosis* and *Plasmodium falciparum) and

[ccp4bb] EMBO Practical Course CEM3DIP 2018: of macromolecular assemblies and cellular tomography: Final Announcement

*Final Announcement* *ONLY ONE DAY LEFT for online application :* *There is no fees for online application. Only chosen participants will have to pay registration fees. Applicants from all over the world are eligible to apply. Applicants from EMBC member states (http://www.embo.org/about-e

[ccp4bb] EMBO Practical Course CEM3DIP 2018: of macromolecular assemblies and cellular tomography

*Second Announcement:* *Applicants from all over the world are eligible to apply. Applicants from EMBC member states (http://www.embo.org/about-embo/member-states ) are in particular encouraged to apply.* *EMBO Practical course Cryo Electron Mic

[ccp4bb] EMBO Practical Course CEM3DIP 2018: of macromolecular assemblies and cellular tomography

*First Announcement:* *EMBO Practical course Cryo Electron Microscopy and 3 Dimensional Image Processing (CEM3DIP 2018): **of Macromolecular assemblies and Cellular tomography* *18-29 March, 2018, at IIT Delhi, New Delhi, India.* The *EMBO Practical Course *Cryo Electron Microscopy a

Re: [ccp4bb] on the resoution of crystal

Dear John, In protein crystallography, the ranges customarily applied to resolution are: Low resolution --> worse than 2.7 A Medium resolution --> better than 2.7 A but worse than 1.8 A High resolution --> better than 1.8 Aand worse than 1.2 A Atom