Re: [ccp4bb] issues

ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 <https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1> -- ----- PD Dr. Peer Mittl Department of Biochemistry Room 44M03 University Zürich Winterthurerstr. 190 8057 Zürich Switzerland Pho

Re: [ccp4bb] ISO model with large groups of atoms in alternative conformations

1=CCP4BB&A=1> -- ****** PD Dr. Peer Mittl Biochemisches Institut Universität Zürich 8057 Zürich Switzerland Phone: +41-44-635 6559 To unsubscribe from the CCP4BB list, click the fo

Re: [ccp4bb] Antwort: [ccp4bb] AW: [ccp4bb] AW: [ccp4bb] Antwort: Re: [ccp4bb] chain on 2-fold axis?

extra molecule. P1 sg looks the only correct option here in my humble opinion. Democracy (voting) depends on science. However, the reverse is not, thankfully. Vaheh From: CCP4 bulletin board <mailto:CCP4BB@JISCMAIL.AC.UK>> On Behalf Of Peer Mittl Sent: Friday, August 27, 2021 6:32 AM T

[ccp4bb] Antwort: Re: [ccp4bb] AW: [ccp4bb] Antwort: Re: [ccp4bb] chain on 2-fold axis?

xtra molecule. P1 sg looks the only correct option here in my humble opinion. Democracy (voting) depends on science. However, the reverse is not, thankfully. Vaheh From: CCP4 bulletin board On Behalf Of Peer Mittl Sent: Friday, August 27, 2021 6:32 AM To: CCP4BB@JISCMAIL.AC.

Re: [ccp4bb] AW: [ccp4bb] Antwort: Re: [ccp4bb] chain on 2-fold axis?

is the “true” space group then P32, with 5 twinned chains, or P3221 with 4 normal chains and 1 chain on a special position? I would vote for the latter. Best, Herman *Von:* CCP4 bulletin board *Im Auftrag von *Peer Mittl *Gesendet:* Freitag, 27. August 2021 10:17 *An:* CCP4BB@JISCMAIL.AC.UK *B

Re: [ccp4bb] Antwort: Re: [ccp4bb] chain on 2-fold axis?

Thu, 26 Aug 2021 at 16:52, Eleanor Dodson mailto:eleanor.dod...@york.ac.uk>> wrote: Great, motto. I think you have nailed it! Did you use tefmac for twinned refinement? And if so what did it suggest the twin  fraction is? On Thu, 26 Aug 2021 at 16:30, Peer Mittl mailto:mi.

[ccp4bb] Antwort: Re: [ccp4bb] chain on 2-fold axis?

you run that locally, you can make refmac do twin refinement. For all resulting structures, I'd also feed the resulting Fcalc (!) into pointless. That should reveal that the packing is indeed close to P3221. Best wishes, Kay On Thu, 26 Aug 2021 11:54:06 +0200, Peer Mittl wrote: >Der

[ccp4bb] chain on 2-fold axis?

Der CCP4 community, Is there a refinement program that can handle protein monomers sitting on crystallographic 2-folds? This is probably a strange question but we have the following situation. We have a 2.6 Ang datasets in SG P3221 (Rpim=4%, Isa=19.6) and a clear molrep solution with 2 chain

Re: [ccp4bb] unknown density in 1.6 A structure

Dear Nick, I like your idea and your comments on this substance. 1,2-hexandiol supposed to be chiral but probably it is used as a racemic mixture in cosmetics. There is even a crystal structure in the PDB (4EUS) and an associated 3 letter code. I´ll give it a try because it seems to fit nicel

[ccp4bb] Powder diffraction database

Could someone please give me some advice on how to query a publicly available powder diffraction database? Upon (protein) crystallization we always get large spherulites of an inorganic compound and I would like to know what it is. It should be possible to use the scattering angles of these sph

[ccp4bb] Density questionable?

Dear Colleagues, We are working on a structure where the density for a whole protein chain (>200 aa) is questionable, since the B-factors exceed 200 Å2 (2.3 Ang resolution). However, the initial difference density map and the feature enhanced map (normal 2fo-fc map to a minor extend) support t

Re: [ccp4bb] unidentified crescent-shaped electron density

rom the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 -- ******** Peer Mittl, PD Dr. Biochemisches Institut Universität Zürich Room 44M03 Winterthurer Strasse 190 CH-8057 Zürich Tel. +41-(0)44-6356559 Mobile +41

[ccp4bb] PhD student position in structure-guided protein engineering at the University of Zürich

We have an immediate opening in the Plückthun lab at the Department of Biochemistry (University of Zürich, Switzerland) for a PhD student to become part of the protein engineering team on the structure-guided development of designed Armadillo-repeat proteins. The lab seeks a talented and motiv

Re: [ccp4bb] self-rotation in the absence of NCS

eresting domain couldnt just be cleaved off could it? Eleanor On 29 April 2015 at 12:59, Peer Mittl mailto:mi...@bioc.uzh.ch>> wrote: We are working with a multi-domain protein crystallized in SG P6_5 with one molecule per asymmetric unit. The structure was refined at 2.00 A resol

Re: [ccp4bb] self-rotation in the absence of NCS

pril 2015 at 12:59, Peer Mittl <mailto:mi...@bioc.uzh.ch>> wrote: We are working with a multi-domain protein crystallized in SG P6_5 with one molecule per asymmetric unit. The structure was refined at 2.00 A resolution with reasonable R-factors but unfortunately the domai

[ccp4bb] self-rotation in the absence of NCS

sn't reveal the "lost" domain. -- ******** Peer Mittl, PD Dr. Biochemisches Institut Universität Zürich Room 44M03 Winterthurer Strasse 190 CH-8057 Zürich Tel. +41-(0)44-6356559 Mobile +41-(0)76-2776566 Fax. +41-(0)44-6356834 Mail mi...@bioc.uzh.ch

[ccp4bb] Refmac error

Dear Colleagues, For some reason Refmac refuses to read the file "mon_lib_list.cif" athough the file exists. The error message is as follows:  Open failed: Unit:   7, File: /share/prog64/ccp4-6.3.0/lib/data/monomers/list/mon_lib_list.cif (logical: /share/prog64/ccp4-6.3.0/lib/data/monomers/l

[ccp4bb] PhD position

At the Department of Biochemistry (University of Zürich) we have an immediate opening for a PhD student working in the field of protein engineering and structural biology. The successful candidate will joint a multidisciplinary team of scientists from combinatorial biochemistry and protein engi

[ccp4bb] P-CUBE workshop on mammalian expression technologies in Oxford

On behalf of the P-cube management team: -Peer Dear All, Don't miss out on the P-CUBE workshop in Oxford in April 2011! Register today under www.p-cube.eu and learn everything about mammalian expression technologies. The registration deadline is March 19, 2011. See you in Oxford! P-CUBE Man

[ccp4bb] P-CUBE - Last chance to sign up for the Workshop and 1st Annual Meeting in Grenoble- Registration is closing soon!

Dear All, Don't miss out on the P-CUBE meeting in Grenoble in September! Sign up today underwww.p-cube.eu. The registration deadline is June 30. 2010. See you in Grenoble P-CUBE Management Team *** Dr. Jutta Tatzel Program Manager P-CUBE Department of Biochemistr

[ccp4bb] P-CUBE: Workshop and 1st Annual Meeting in Grenoble- Registration is closing soon!

On behalf of the P-cube Management Team Dear All, To make sure you don't miss out on the P-CUBE meeting in Grenoble in September, please sign up as soon as possible under*www.p-cube.eu*. The registration deadline is June 30. 2010. See you in Grenoble P-CUBE Management Team

[ccp4bb] Pcube User meeting

Dear Colleagues, I would like to draw your attention to the upcoming Pcube-user meeting (September 8-9, 2010 in Grenoble). You are probably aware that the EU-founded Pcube project provides open access to several supportive technologies for macromolecular crystallography, ranging from fragment

[ccp4bb] Postdoctoral position at UZH on designed repeat proteins

under: http://www.bioc.uzh.ch/index.php?id=3&L=1 -- **** Peer Mittl, PD Dr. Biochemisches Institut Universität Zürich Winterthurer Strasse 190 CH-8057 Zürich Tel. +41-(0)44-635 6559 Fax. +41-(0)44-635 6834 Mail mi...@bioc.uzh.ch

[ccp4bb] Open Post-Doc and technician positions

the Post-Doc positions) and the names of two to three referees to Mrs. Salome Rittmeyer at the following address: secgruet...@bioc.uzh.ch Best regards, Peer Mittl vacantPosition_UZH.pdf Description: Binary data

[ccp4bb] 21st Rhine-Knee Regiomeeting 2007

Registration for the 21st Rhine-Knee Regiomeeting 2007 in Hölstein (CH) is open until August 31th, 2007 under http://www.bioc.uzh.ch/index.php?204&L=1 . Best regards, Peer Mittl