Hi Rohit,
A nice tool I came across recently, other than PISA, was EPPIC:
http://eppic-web.org/ewui/ .
This tool analyses the interface in a similar way to PISA, looking at size
and gives a per-residue percentage buried surface area. It also does an
evolutionary analysis to determine how
Hi Chiara,
We also have an Avant at room temperature. My recommendation would be to
forgo using a water bath. Instead you can achieve a prep near enough to 4C
by doing 2 things: Firstly, keep your columns stored in the cold room or a
fridge to pre-chill them before use. Secondly, make your
This short paper in Nature Methods is probably worth a read and may
help with your Ni-NTA issues:
http://www.nature.com/nmeth/journal/v6/n7/full/nmeth0709-477.html
Nature Methods 6, 477 - 478 (2009)
doi:10.1038/nmeth0709-477
Enabling IMAC purification of low abundance recombinant proteins from
(just forwarding this to the list...)
Dear Tim,
I think it may be possible, though rather long-winded to do. First of all,
you'll need a working installation of mediawiki. My suggestion to get this
would be to download one of the self-contained installations available from
bitnami:
Hi there,
I just got this working a couple of days ago under Mac OS X 10.6.3. I
manually compiled and installed Perl-Tk from CPAN. Steps taken:
1. Download and ungzip / untar:
http://search.cpan.org/CPAN/authors/id/S/SR/SREZIC/Tk-804.028_501.tar.gz(the
newer -503 version didn't work)
2. Change
: VectorNTI alternatives - 4 Mac?
From: Mark Collins mcoll...@convex.hhmi.columbia.edu
To: CCP4BB@JISCMAIL.AC.UK
Date: Wed Jan 28 15:02:17 2009
Hi
Anybody have suggestions for Mac OsX alternatives?
Thanks in advance,
Mark
--
Roger Dodd PhD
The Institute of Cancer Research
Chester Beatty Laboratories
?
Thanks in advance,
Xie
--
Roger Dodd PhD
The Institute of Cancer Research
Chester Beatty Laboratories
237 Fulham Road
London
SW3 6JB
UK
The Institute of Cancer Research: Royal Cancer Hospital, a charitable Company
Limited by Guarantee, Registered in England under Company No. 534147 with its
