Re: [ccp4bb] Strange density on Serine oxygen.

glycosylation. All the best, Vinson  发件人： Savvas Savvides savvas.savvi...@ugent.be 收件人： Vinson LIANG lwg_conrad_1...@yahoo.com.cn 抄 送： CCP4BB@JISCMAIL.AC.UK 发送日期： 2010/11/24 (周三) 9:27:31 下午 主 题： Re: [ccp4bb] Strange density on Serine oxygen. Hi Vinson Beyond

Re: [ccp4bb] Strange density on Serine oxygen.

savvas.savvi...@ugent.be 收件人： Vinson LIANG lwg_conrad_1...@yahoo.com.cn 抄 送： CCP4BB@JISCMAIL.AC.UK 发送日期： 2010/11/24 (周三) 9:48:28 下午 主 题： Re: [ccp4bb] Strange density on Serine oxygen. Hi Vinson is O-glycosylation possible at all given the origin of the protein and the expression system used?  best  Savvas

Re: [ccp4bb] Strange density on Serine oxygen.

/databases/OGLYCBASE/). best regards Savvas Savvas Savvides Unit for Structural Biology @ L-ProBE Ghent University K.L. Ledeganckstraat 35, 9000 Ghent, Belgium Ph. +32  (0)472 928 519 http://www.LProBE.ugent.be/xray.html On 24 Nov 2010, at 13:10, Vinson LIANG wrote: Dear all, I'm

Re: [ccp4bb] R-free flag problem

： Vinson LIANG lwg_conrad_1...@yahoo.com.cn 发送日期： 2010/10/22 (周五) 12:44:58 上午 主 题： Re: [phenixbb] R-free problem with phenix.refine Hi, Recently I deposited a structure to PDB. However, I was told The R value of SF check are different from your reported value. R work in SF check = 0.2859

Re: [ccp4bb] R-free flag problem

+0800, Vinson LIANG wrote: Dear all, Thank you very much for your suggestions. I try Pavel's easy method. Hopefully, the result from phenix.model_vs_data would be enough for PDB.   Best wishes,   Vinson     发件人： Pavel Afonine pafon

[ccp4bb] R-free flag problem.

not recognize. Could anyone please help me to solve this problem? Thank you very much in advance, Best, Vinson, Liang

Re: [ccp4bb] Is it possible to mutate a reversible epimerase into an inreversible one?

of the reaction. So I think the best thing I could do is to take Mickael's suggestion and add another enzyme to change the concentration of one substrate.   However, Lijun's comment do open my mind.   I really appreciate all your help,   Best Regards,   Vinson Liang  --- 10年5月22日，周六, Dale

[ccp4bb] Is it possible to mutate a reversible epimerase into an inreversible one?

Dear all,   Sorry for this silly biochemistory question.  Thing is that I have a reversible epimerase and I want to mutate it into an inreversible one. However, I have been told that the ΔG of a reversible reaction is zero. Which direction the reaction goes depends only on the concentration of

[ccp4bb] How to generate lib file for new ligand?

, Vinson Liang