Hi all,
We're planning to use short oligos to co-crystallize with protein.
Basically we will synthesis that from company and then purified by
ion-exchange then concentrate it. Does any one know a easy way or product
to concentrating short oligo (such as 5nt)? The only way came to my mind,
is to de
o
2014-10-13 17:59 GMT-07:00 Mayer, Mark (NIH/NICHD) [E]
:
> Try either glycerol or PEG 400.
> Increasing concentration of 'high' MW PEG is not a good strategy.
>
> ____
> From: Xiao Xiao [victor41...@gmail.com]
> Sent: Monday,
Hi,
Since my crystallization condition contains PEG, I am trying to use PEG as
one of my cryo for testing. For example, one crystallization condition is
0.2M Na2PO4, 20%PEG3350. I want to make 0.2M Na2PO4 and 40% PEG3350 as
cryoprotectant. When I added 200ul salt from 1M stock, and 800ul PEG from
did mention, in a few cases, some protein crystals were hard
to dissolve.
5. Using low pH(3-5), NaOH, or simply add SDS loading buffer and heat may
help dissolve the crystal.
Again, thanks a lot for all your help!
Best regards,
Xiao Xiao
2014-09-25 16:53 GMT-07:00 Xiao Xiao :
> Hi every
Hi everyone,
Sorry for an off-topic question.
I got a problem with crystal dissolving. Basically I got crystals of my
protein in various conditions, most conditions contain PEGs but different
salts. These crystals has very similar shape, so it should not be salt.
Now I am trying to dissolve the
for my purpose. I'll discuss with
my colleague to decide which model we are gonna purchase. Again thank you
all for your suggestion!
Best,
Xiao
2014-06-09 14:41 GMT-07:00 Xiao Xiao :
> Dear all,
>
> Here's a very basic question --
> I am doing some initial crystall
Dear all,
Here's a very basic question --
I am doing some initial crystallization screening now but unfortunately our
robot got broken and it will take a while to get funded for buying a new
one:( So I have to set up all trays by hand. While it is still durable to
use our current pipettes sets, wh
lways prefer to use expression systems that’s most close to
> the protein’s natural habitat. There are proteins that would never be folded
> in E coli.
>
> Zhijie
>
>
>
> From: Xiao Xiao
> Sent: Thursday, November 14, 2013 2:18 AM
> To: CCP4BB@JISCMAIL.AC.UK
> S
t;3hr at 37C) or harsh lysis can
> contribute to misfolding.
>
> One more question: does your protein contain cys residues? Are they
> supposed to be oxidized or reduced?
>
> Zhijie
>
>
>
> -Original Message- From: Xiao Xiao
> Sent: Wednesday, November 13, 2
can try in order to eliminate heterogeneity?
Any suggestion or comment will be really appreciated.
Thank you!
Best regards,
Xiao Xiao
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