[ccp4bb] What's happened over the last five years with high-throughput protein crystallization screening?

Dear CCP4BB Community, I would like to comment you guy a review of the last five years of high-throughput protein crystallization screening that would be a magnificent help for all scientists that struggle with macromolecular crystallization. Please see attachment, as well as below:

Re: [ccp4bb] What's happened over the last five years with high-throughput protein crystallization screening?

mia and industry. Hopefully the cost of the robotic equipment > will come down. > > -Daniel > > > On Thu, Apr 26, 2018 at 11:43 AM, Yibin Lin <yyb...@gmail.com> wrote: > >> Dear CCP4BB Community, >> >> I would like to comment you guy a review of the last

[ccp4bb] crystals disappear

Hi forks, I got some membrane protein crystals in DDM. However, after one month, these crystals change to Irregular shape, and have not birefringent in polarized Light. Do anybody know why? Does the effect of DDM? Contain too much detergent? Thanks, Zhang Peng

[ccp4bb] Crystals

Hi fork, I am working with a membrane protein. I got some crystals from peg400. All of these crystals don't diffract. I tried to use a needle to break crystal. They didn't break into small one, look like gel, or oil, something like that. Do anybody has deal about these crystals. Detergent

[ccp4bb] Expression of a Selenomethionine Variant in E. coli

Hi List, I want to preprare sel-met labeling protein. Could someone can tell me if it is necessary to add DTT or BME to medium, which contains sel-met, when growing cells? Thanks in advance, Yibin Lin

Re: [ccp4bb] setting up additive screen

Use robot. You only need 0.1ul*96=9.6ul of protein solution. On Thu, Mar 3, 2011 at 7:59 PM, m zhang mzhang...@hotmail.com wrote: Dear all, I am trying to optimize my crystal with additives. Since the yield of my protein purification is very limited, I am wondering what is the most

Re: [ccp4bb] Could someone can help me to explain why EDTA-2Na can formate salt crystals

if you have a very small amount present, say as a contaminant with citrate or EDTA, it will crystallize. On Feb 21, 2011, at 1:22 PM, Yibin Lin wrote: Dear all, I got a lot of salt crystals in reservior solution (well solution), which contains 0.1 M phosphate/citrate ph 4.2, PEG200 47

[ccp4bb] Could someone can help me to explain why EDTA-2Na can formate salt crystals

Dear all, I got a lot of salt crystals in reservior solution (well solution), which contains 0.1 M phosphate/citrate ph 4.2, PEG200 47%, EDTA-2Na 0-22mM. Reservior solution appears crystals from 12mM EDTA. Could someone help me to explain why? Thank you very much! Yibin

Re: [ccp4bb] low expression and aggregation of protein

Actually, it is very difficult to say something just based on your simple information. You should give more informations about your work, for example, temperature, cuture, etc. Best, Lin On Fri, Jan 21, 2011 at 8:12 PM, m zhang mzhang...@hotmail.com wrote: Dear All, Recently I am trying to

[ccp4bb] selenomethinine-labeled protein

Dear all, I try to express selenomethinine-labeled protein in E.coli methinoine auxotrophic strain B834 in M9 with 17 amino acid and 50 mg/ml se-met for 18 hours at 37 degree. I don't why the colour of medium vary from colorless to yellow. Could anybody tell me it is normal or not? Thanks a lot!