Re: [ccp4bb] crystallization of a macromolecular complex

2010-03-06 Thread bas
Dear Leo, here's a little gedankenexperiment: imagine cross-linking those solutions you have, say with glutaraldehyde. Would you say this will *improve* solubility because there are now less molecules? Best wishes, Sebastiaan Werten. Dear all -- I have a very stupid question/remark

Re: [ccp4bb] [off-topic] new initiative for science TV

2009-10-10 Thread bas
We all love science Fred, but are you sure you would want to encourage young Frenchmen to start a career in it? After all, one's own glossy TV channel is poor compensation for a lifetime of precariousness. I'm afraid that if people in France choose to do an MBA instead, right now that may not be

Re: [ccp4bb] Phasing at Low Resolution

2009-05-15 Thread bas
Dear Clemens, sounds like typical pathology of plate-like crystals, which often suffer from slight growth defects and possibly deformation during handling/freezing. If the Se-Met crystals are indeed easy to obtain in large numbers you may want to test as many of them as you can, because there

Re: [ccp4bb] problem in transformation of pqe 30 clone

2009-05-04 Thread bas
This story is rather puzzling indeed, and it is difficult to see why in case of toxicity one would have transformation problems with that one strain in particular. Also, I am wondering how likely it is that a combination of toxicity and leaky expression would lead to transformation problems in

[ccp4bb] Software patents

2009-04-06 Thread bas
Dear colleagues, it appears that following the rejection of the software patent directive by the European Parliament there is now another attempt going on to get software patents consolidated, this time through the back door. Since the threat of software patents is relevant to the

Re: [ccp4bb] Post-doc position at the IBS, Grenoble, France

2008-01-04 Thread bas
- Original Message - From: Dominique BOURGEOIS To: CCP4BB@JISCMAIL.AC.UK Sent: Friday, January 04, 2008 4:18 PM Subject: [ccp4bb] Post-doc position at the IBS, Grenoble, France Kinetic protein crystallography of fluorescent proteins. [...] Candidates should have a strong expertise in

Re: [ccp4bb] To bathe or not to bathe.

2007-11-26 Thread bas
- Original Message - From: Nave, C (Colin) [EMAIL PROTECTED] Several have raised the issue of radiation damage. The strategy which Bob mentions can make sense, ensuring fresh parts of the crystal are regularly brought in to the beam. I would have thought 5-10 micron beams were rather