[ccp4bb] How to know if ADP exists in the ATP-binding site of bacterial expressed proteins

Deer CCP4ers, How to know if ADP exists in the ATP-binding site of bacterial expressed proteins? Check the UV spectrum at 280 nm is one way, but there is no Trp in my protein. Is there any other conveniet way to find out? Thanks in advance Best wishes Xinghua Qin -- Xinghua Qin State

[ccp4bb] how to ignore spot overlap in imosflm?

. Although the completeness is quite low (just 50%), now I have determined the structure (Rfree=0.31, Rfactor=0.24, resolution=2.6). I will look into the map with COOT, and try to get the structure more beautiful. Thanks again Best wishes Xinghua Qin -- Xinghua Qin State Key Laboratory of Plant

[ccp4bb] about the working solution

to be the working solution? In my opinion, the latter one has fewer nucleus, but the former one has better shape, did I got the point? Best wishes Xinghua Qin -- Xinghua Qin Room 4022, Center for Life Sciences, China Agricultural University, No.2, Yuan Ming Yuan West Road, Haidian District， Beijing

[ccp4bb] about partial plroteolysis

hello CCp4ers: Sorry about the off-topic question. My protein is a kinase which the predicted kinase domain is from 32aa to 319aa. But the protein sequence after partial proteolysis is from 52aa. what should I do? Are there any reasonable expanation? Thanks in advance! Xinghua Qin -- Xinghua

[ccp4bb] how to fix a single helice

Hi CCP4ers: Recently I am trying to fix a helice, but found that it is so hard for me because of the poor resolution(3.2A) and also a unvisible loop near it, so is there any way to just fix a helice ? can I get it out of the whole structure to refine it? Best regards -- Xinghua Qin College

[ccp4bb] Matthews coefficient

, Then I used two molecules in the unit cell, After refining with Refmac, I found that the R factor is 0.29, R free is 0.40.I believe the value can be better with the real space refinement.But the question is that can the calculated Matthews coefficient be wrong? Best regards Xinghua Qin

Re: [ccp4bb] Matthews coefficient

Xinghua Qin On Fri, Jun 25, 2010 at 10:42 AM, Vineet Gaur vineetgaur1...@gmail.comwrote: Hi It would b good if u can mention the solvent content and space group along with the no. of molecules in asu/ u can carry out self rotation function to check the no of mol in asu best vineet On Fri, Jun