As I understood the problem, it is that automatic assignment comes out
differently for different structures of the same protein or proteins so close
that they should have the same secondary structure, due to differences in
quality of the structures. The question then is not how to determine
Dear J. Vitali,
there are tools that have been mentioned by others, that assist with the
assignment. The final decision depends on you as researcher. You should
visually check the hydrogen bonding whether the boundaries are consistent with
the automated assignment.
People often seem to think