Hi there,
Was recently looking at a structure of an enzyme with AMP-PNP added to
the crystallization mix, and all I see is density for ADP. I was
wondering if hydrolysis of AMP-PNP to ADP is relatively common - either
as a result of extended time in crystallization or exposure of the
resultant
Hi Steve,
With my experience, it is (very) common to see AMPPNP is hydrolyzed to AMPPN
(supposedly) with my protein. Although the literature often reported AMPPNP
as a stable ATP mimic, such a luck wasn't true with my case, maybe same as
you. If you go to Sigma website where I purchased, it may
Hi Steve,
Funnily enough I just read the following paper today, which describes exactly
this phenomenon:
http://www.ncbi.nlm.nih.gov/pubmed/21093442
Is AMPPCP as sensitive to acid conditions? I would suspect not.
Best wishes
Derek
: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of
Derek Logan
Sent: Monday, February 14, 2011 4:16 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] AMP-PNP Hydrolysis
Hi Steve,
Funnily enough I just read the following paper today, which describes
exactly this phenomenon:
http
, February 14, 2011 4:16 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] AMP-PNP Hydrolysis
Hi Steve,
Funnily enough I just read the following paper today, which describes exactly
this phenomenon:
http://www.ncbi.nlm.nih.gov/pubmed/21093442
Is AMPPCP as sensitive
Is it possible that the phosphates are just disordered rather than being
cleaved? It's always the case for inactive kinase-ATP or AMPPNP complexes
that the phosphates are not stabilized by Mg2+ or the residues in the
binding pocket and hence they become disordered and are not seen in the
electron
Once again, I've sent off a message only to one person that was intended for
the BB.
Begin forwarded message:
From: Charles W. Carter, Jr car...@med.unc.edu
Date: February 14, 2011 6:23:16 PM EST
To: Martin Picard martin.picard...@gmail.com
Subject: Re: [ccp4bb] AMP-PNP Hydrolysis
I take
