Auftrag von Tom Burnley -
> UKRI STFC
> Gesendet: Mittwoch, 29. Juli 2020 18:51
> An: CCP4BB@JISCMAIL.AC.UK
> Betreff: Re: [ccp4bb] AW: [EXTERNAL] Re: [ccp4bb] real real-space-refinement
>
> EXTERNAL : Real sender is owner-ccp...@jiscmail.ac.uk
>
>
>
> Hi Herman
: 30 July 2020 13:28:00
To: ccp4bb
Subject: [ccp4bb] AW: [ccp4bb] AW: [EXTERNAL] Re: [ccp4bb] real
real-space-refinement
Hi everybody,
Thank you very much for the many tips! I discovered that in Coot by clicking on
the N- and C-terminus of a protein chain, one can indeed run a real-space
Nachricht-
Von: CCP4 bulletin board Im Auftrag von Tom Burnley -
UKRI STFC
Gesendet: Mittwoch, 29. Juli 2020 18:51
An: CCP4BB@JISCMAIL.AC.UK
Betreff: Re: [ccp4bb] AW: [EXTERNAL] Re: [ccp4bb] real real-space-refinement
EXTERNAL : Real sender is owner-ccp...@jiscmail.ac.uk
Hi Herman,
The
etin board Im Auftrag von Tom Burnley -
UKRI STFC
Gesendet: Mittwoch, 29. Juli 2020 18:51
An: CCP4BB@JISCMAIL.AC.UK
Betreff: Re: [ccp4bb] AW: [EXTERNAL] Re: [ccp4bb] real real-space-refinement
EXTERNAL : Real sender is owner-ccp...@jiscmail.ac.uk
Hi Herman,
There is lots of information o
Hello,
Have you tried ISOLDE? Interactive MDFF is really powerful both for fixing
models with poor geometry and for improving fit to the map.
There is a good tutorial for refinement of a model against a cryoEM map with
ISOLDE:
(and
runs Refmac via CCP4).
All the best,
Tom
From: CCP4 bulletin board on behalf of Boaz Shaanan
Sent: 29 July 2020 16:31:46
To: ccp4bb
Subject: Re: [ccp4bb] AW: [EXTERNAL] Re: [ccp4bb] real real-space-refinement
Hi,
Try searching ccp-em.
Boaz
Boaz Shaanan
Hi,
Try searching ccp-em.
Boaz
Boaz Shaanan, Ph.D.
Department of Life Sciences
Ben Gurion University of the Negev
Beer Sheva
Israel
On Jul 29, 2020 18:29, "Schreuder, Herman /DE" wrote:
Coot does it, but if one wants to do it for a complete protein, it is a lot of clicking. On the
Coot does it, but if one wants to do it for a complete protein, it is a lot of
clicking. On the other hand, I have not tried to just select the N- and
C-terminus for real-space refinement to see what happens. I would prefer to
have some script to do it.
Best, Herman
Von: Eleanor Dodson
