[ccp4bb] AW: [EXTERNAL] [ccp4bb] Assumptions on protein purification

ERNAL] [ccp4bb] Assumptions on protein purification Dear all, Not directly a ccp4 question. I am working on a multi-domain protein with multiple catalytic centres. Purifying in gel-filtration (Äkta) with different buffers gives a clear distinct peak indicating pure protein. We could even crystallise i

[ccp4bb] Assumptions on protein purification

Dear all, Not directly a ccp4 question. I am working on a multi-domain protein with multiple catalytic centres. Purifying in gel-filtration (Äkta) with different buffers gives a clear distinct peak indicating pure protein. We could even crystallise it and determine 3D structure to about 2.5 A. Why