One other staining method deserves to be mentioned with the others
already in this thread:
We use standard issue Coomassie Blue staining, but do the staining and
destaining with heated solutions (place the gel in a tray on a hotplate
set to ~90drg in a fumehood). We routinely have the result
You can also do this more quickly by microwaving your gels in
stain/destain solutions
just for the lazy or impatient
Regards
Mike
On Wed, 2008-03-05 at 12:09 +0100, Bjørn Pañella Pedersen wrote:
One other staining method deserves to be mentioned with the others
already in this thread:
We
Dear Crystallographers:
just to share something I found out recently, that is really helpful to
know:
5 min Copper Stain protocol
Normal SDS-PAGE gels can be stained in 5 min with 300mM CuCl2. Simply remove
the gel, rinse ~10s in water, place 5 min in CuCl2, and the gel is
negatively
: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On Behalf Of
Jacob Keller
Sent: Tuesday, March 04, 2008 12:03 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Copper Staining
Dear Crystallographers:
just to share something I found out recently, that is really helpful to
know:
5 min Copper Stain
, 2008 6:03 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Copper Staining
Dear Crystallographers:
just to share something I found out recently, that is really helpful to
know:
5 min Copper Stain protocol
Normal SDS-PAGE gels can be stained in 5 min with 300mM CuCl2. Simply remove
the gel, rinse
]
***
- Original Message -
From: Artem Evdokimov [EMAIL PROTECTED]
To: 'Jacob Keller' [EMAIL PROTECTED];
CCP4BB@JISCMAIL.AC.UK
Sent: Monday, March 03, 2008 6:31 PM
Subject: RE: [ccp4bb] Copper Staining
There's an even faster reagent for staining - takes abut 1 minute
There's an even faster reagent for staining - takes abut 1 minute overall
and the results look just like dear old Coomassie. This reagent works
wonderfully for us. Yes, the recipe is proprietary - but if you add up the
cost of reagents for normal Coomassie and compare - this one isn't much more
