Hello Careina,
In addition to all the good suggestions you already got, I can point to these
very good references:
- Pryor EE Jr, Wozniak DJ & Hollis T (2012) Crystallization of Pseudomonas
aeruginosa AmrZ protein: development of a comprehensive method for obtaining
and optimization of protein
Carina, to complement the techniques using sticky ends, etc., you can also
use the "random" microseeding approach that I mentioned to Kavya, see below.
The great advantage in a project like yours, where you have a family of
related constructs, is that you can use cross-seeding - that is, you can
u
Hello,
Overhanging "sticky" ends are mentioned frequently when it comes to
obtaining infinite helices that are useful in crystallization. For
example in
https://home.ccr.cancer.gov/csb/nihxray/Tips-and-Tricks_Crystallization_Protein-DNA_updated.pdf
.
Cheers,
Fred.
On 09/02/2024 10:59, car
Hello,
If you want to promote "infinite" helix, you should go for overhangs
(sticky end) with compatible sequence. I try to explain better what I
have in mind (and I actually did).
example :
5'ATCCCTAAATCGGCGTGTGCT---3'
3'---GGATTTAGCCGCACACGATAG5'
Hoping that this results in something li
Thank you for this insight, Nicolas. It is very helpful.Yes I have also had a
soccer ball shaped crystal that does not diffract as well as, and more
recently, many plate like crystals but they do not diffract either.I do know I
have both protein and DNA in my crystals but I do not know, as you s
Hello Careina,
In my hands, DNA protein complex crystals may be frustrating, because
often we get good looking crystals which don't diffract at all and are
actually not easy to improve.
I remember obtaining a lot of crystal looking a bit like "STOP" road
sign (octogonal shape for one axis) w
Assunto: [ccp4bb] Crystals with DNA
Hello all.
I am struggling to get defracting crystals with a protein DNA complex. The
crystals are plentiful but they do not diffract. I am going back to the grind
stone and relookong at my DNA sequence.
Is there any wisdom you could give me with regards to what
Hello all.
I am struggling to get defracting crystals with a protein DNA complex. The
crystals are plentiful but they do not diffract. I am going back to the grind
stone and relookong at my DNA sequence.Is there any wisdom you could give me
with regards to what works best with DNA in crystals?F
