[ccp4bb] Defining beamstop and error during indexing- moslfm

2012-03-22 Thread sonali dhindwal
Dear All, We have collected a data for a protein crystal at SER-CAT Chicago and the detector is mar300.We are using mosflm to process the data.While indexing,  the beamstop which it is taking is wrong, due to which it fails. I am trying to define the beamstop manually using tools like mask and

Re: [ccp4bb] Defining beamstop and error during indexing- moslfm

2012-03-22 Thread Tim Gruene
-BEGIN PGP SIGNED MESSAGE- Hash: SHA1 Dear, as you open the imosflm-GUI there are three editable fields on top underneath the top menu which are for the beam position (x,y in mm) and the detector distance. As you tell imosflm which images to use, a display window pops open which allows y

Re: [ccp4bb] Defining beamstop and error during indexing- moslfm

2012-03-22 Thread Andrew Leslie
Dear Sonali, Just to add to Tim's reply, when you open the image with iMosflm, you can Drag and drop the direct beam position in the image display window. First, you have to click on the leftmost icon in the row of icons under the image filename (a green cross) which will display the d

Re: [ccp4bb] Defining beamstop and error during indexing- moslfm

2012-03-22 Thread Mark J van Raaij
If you are lucky (or should I say unlucky) and have an ice-ring on the same image or on another image collected during the same shift and similar distance, you can estimate the beam centre using the "fit circle" option. Although software (and beamlines) have improved greatly, it can still be a go

Re: [ccp4bb] Defining beamstop and error during indexing- moslfm

2012-03-22 Thread sonali dhindwal
.” --- On Thu, 22/3/12, Andrew Leslie wrote: From: Andrew Leslie Subject: Re: [ccp4bb] Defining beamstop and error during indexing- moslfm To: CCP4BB@JISCMAIL.AC.UK Date: Thursday, 22 March, 2012, 7:43 PM Dear Sonali,             Just to add to Tim's reply, when you open the image with iMosflm, yo

Re: [ccp4bb] Defining beamstop and error during indexing- moslfm

2012-03-22 Thread Francis E Reyes
What resolution are you working at? What are the unit cell dimensions? On Mar 22, 2012, at 11:10 AM, sonali dhindwal wrote: > Dear Andrew and all the people for their help, > > I am providing mosflm the right beamstop and now, I am able to do the > indexing, refinement and indexing too. > Then

Re: [ccp4bb] Defining beamstop and error during indexing- moslfm

2012-03-22 Thread Andrew Leslie
2, Andrew Leslie wrote: > > From: Andrew Leslie > Subject: Re: [ccp4bb] Defining beamstop and error during indexing- moslfm > To: CCP4BB@JISCMAIL.AC.UK > Date: Thursday, 22 March, 2012, 7:43 PM > > Dear Sonali, > >             Just to add to Tim's reply, when y