Re: [ccp4bb] Dehydration treatments

2011-05-06 Thread Derek Logan
Hi, Yes indeed, as Matt was kind enough to point out, we do have the HC1 installed at station I911-3 of the MAX-II ring and you are welcome to make a rapid access application. One advantage of MAX-lab might be that our wiggler beam is more merciful to the crystals at room temperature than the

Re: [ccp4bb] Dehydration treatments

2011-05-03 Thread Michael Thompson
...@gmail.com To: CCP4BB@JISCMAIL.AC.UK Sent: Sunday, May 1, 2011 10:32:21 AM GMT -08:00 US/Canada Pacific Subject: [ccp4bb] Dehydration treatments Hi folks, I am currently impressed by the efficiency of dehydration treatments over the diffraction capacity of our crystals in one particular condition

Re: [ccp4bb] Dehydration treatments

2011-05-02 Thread Matthew BOWLER
Dear Israel, as Martin pointed out we have a device here at the ESRF/EMBL, the HC1b, that produces a stream of air with a precisely controlled RH at the sample position that we have used with some success to monitor the effects dehydration has on diffraction quality. The same device is

Re: [ccp4bb] Dehydration treatments

2011-05-01 Thread Horacio Botti
Hi Israel! The phenomena you describe seems to be related to cryoprotection, but you don't say anything about collection temperature or cryoprotection used. Controlled-slow dehydration is one way of achieving optimized cryocooling of specially fragile crystals. I would recommend you to

Re: [ccp4bb] Dehydration treatments

2011-05-01 Thread Gerard Bricogne
Dear Israel, There is a lot to look up in this field. I believe it started with Schick, B. Jurnak, F. (1994). Acta Cryst. D50, 563-568. at http://scripts.iucr.org/cgi-bin/paper?gr0289 There is a nice review online at

Re: [ccp4bb] Dehydration treatments

2011-05-01 Thread Martin Weik
Dear Israel, there is also a dedicated humidity device developed at EMBL/ESRF Grenoble that allows for systematic dehydration experiments to be carried out at room temperature and soon at 4°C. Here is the primary reference: Sanchez-Weatherby, J., Bowler, M.W., Huet, J., Gobbo, A., Felisaz,

Re: [ccp4bb] Dehydration treatments

2011-05-01 Thread David Waterman
Hi Israel, Just to add to that, and as I know you use the Diamond synchrotron, the device mentioned is available (on advance request) on the beamline I02. Cheers -- David On 1 May 2011 19:50, Martin Weik martin.w...@ibs.fr wrote: Dear Israel, there is also a dedicated humidity device

Re: [ccp4bb] Dehydration treatments

2011-05-01 Thread Catarina Rodrigues
Dear Israel, This paper describes a procedure to crystal desiccation that allowed the improvement of diffraction: C. Abergel Acta Cryst. (2004). D60, 1413-1416 http://scripts.iucr.org/cgi-bin/paper?S0907444904013678 HTH Good luck, Catarina -- Catarina Rodrigues, PhD Student Molecular