Re: [ccp4bb] Difficult data

2013-04-18 Thread Andrey Nascimento
CCP4BB@JISCMAIL.AC.UK Sent: Tue, Apr 16, 2013 7:39 pm Subject: [ccp4bb] Difficult data Hello, I am having a few issues with a data set I have been working on recently, and was hoping to get some ideas on how to deal with it, if anyone is in the mood. I have been working with a very small

Re: [ccp4bb] Difficult data

2013-04-17 Thread Herman . Schreuder
advice would be: go back to the lab and try to get another crystal form. Herman From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Stephen Campbell Sent: Wednesday, April 17, 2013 5:53 AM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] Difficult

Re: [ccp4bb] Difficult data

2013-04-17 Thread mjvdwoerd
, 2013 7:39 pm Subject: [ccp4bb] Difficult data Hello, I am having a few issues with a data set I have been working on recently, and was hoping to get some ideas on how to deal with it, if anyone is in the mood. I have been working with a very small bacterocin (about 3 kDa) and set up some

[ccp4bb] Difficult data

2013-04-16 Thread Stephen Campbell
Hello, I am having a few issues with a data set I have been working on recently, and was hoping to get some ideas on how to deal with it, if anyone is in the mood. I have been working with a very small bacterocin (about 3 kDa) and set up some crystal trays in hope of getting some high

Re: [ccp4bb] Difficult data

2013-04-16 Thread Jim Pflugrath
Maybe you crystallized something else? Did you look up that unit cell in the PDB? I am having a few issues with a data set I have been working on recently, and was hoping to get some ideas on how to deal with it, if anyone is in the mood. .

Re: [ccp4bb] Difficult data

2013-04-16 Thread Edward A. Berry
Yes- this has happened to us so often recently that I've taken to checking every new crystal form against the nearest cell server before starting heavy atom search. That would account for the poor reproducibility. Yeast glutamate-cysteine ligase (e.g. 3LVV) crystallizes with nearly identical

Re: [ccp4bb] Difficult data

2013-04-16 Thread Stephen Campbell
Hi Everybody, Thanks so much for all the help! I suppose I should give more info. Marco, the data is twinned - I forgot about that in my initial posting. I can't remember the stats, but it is not a perfect twin. I thought that Phaser accounted for twinning, and I haven't got a solution good