Gesendet: Tuesday, August 11, 2009 12:06 AM
An: CCP4BB@JISCMAIL.AC.UK
Betreff: [ccp4bb] Disulfide bond "survival" in the presence of DTT?
Hi all-
Got a perplexing thiol chemistry/phasing issue. To prevent non-native
disulfide bonds from forming between free Cys but to preserve native
dis
etin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of Brad
Bennett
Sent: Monday, August 10, 2009 5:06 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Disulfide bond "survival" in the presence of DTT?
Hi all-
Got a perplexing thiol chemistry/phasing issue. To prevent non-native
disulfide bonds
Hi all-
Got a perplexing thiol chemistry/phasing issue. To prevent non-native
disulfide bonds from forming between free Cys but to preserve native
disulfides, I have heard of using a very low (say < 0.5 mM) concentration of
DTT. I've recently come across a paper where the assignment of 3 disulfides
