Hi Jan,
you will find some methods for detection of thiols and/or disulfides there:
http://strucbio.biologie.uni-konstanz.de/ccp4wiki/index.php/Thiols_and_disulfides
HTH,
Guenter
Dear all,
I am working on a protein where I have to stabilize the closed
conformation of the protein using disulp
renzo
Lorenzo Ihsan FInci, Ph.D.Postdoctoral Scientist, Wang LaboratoryHarvard
Medical SchoolDana-Farber Cancer InstituteBoston, MA Peking UniversityThe
College of Life SciencesBeijing, China
Date: Wed, 11 Jul 2012 11:12:17 -0400
From: kellydaugh...@gmail.com
Subject: Re: [ccp4bb] Disulphide bond
Ellman's assay may help you.
http://en.wikipedia.org/wiki/Ellman's_reagent
http://www.piercenet.com/instructions/2160311.pdf
Allan
Quoting Jan Rashid Umar :
Dear all,
I am working on a protein where I have to stabilize the closed conformation
of the protein using disulphide bond. The strateg
Jan,
If the Cys residues are accessible, you could try DTNB to quantify the
number of Cys, thus determining if they are reduced or bridged.
http://en.wikipedia.org/wiki/Ellman's_reagent
Kelly
***
Kelly Daughtry, Ph.D.
Post-Doctoral Fellow, Rae
Dear all,
I am working on a protein where I have to stabilize the closed conformation
of the protein using disulphide bond. The strategy to design the cysteine
mutants is based on the molecular dynamic simulations, and accordingly the
residues were chosen. The ultimate goal is to trap the ligand i
