Tommi,
The question has been asked and answered not by protein
crystallography, but by cyroelectron microscopy and EM freeze etch
research. Even as far back as the early 1960's, people noticed that
liq. N2 was really slow at cooling. Read the cyroEM work on the
bacteriorhodopsin photocyc
according to literature,see below and references
http://www.px.nsls.bnl.gov/courses/papers/ZD_EG_papers.html,
it is not clear that liq. propane plunged item would cool
faster. (whilst i havent tested this)...
Would anyone have actual experimental data with protein crystals
on the hyperquenchin
I think the important thing here is that liquid nitrogen in the lab
tends to be exactly at its boiling point, since the temperature is
maintained by continuously boiling off some of the N2.
This means the only mechanism for heat absorption is through vaporization,
depending on the latent heat of
yes you are right, but I assumed if people see a cloud of condensed
fog over their LN2 bath they should remove that by
a) filling up the bowl completely e.g. some LN2 drips out of the bowl
b) blow the fog away before you dip
I think the original poster meant the relatively low heat conduction of
Hi Charlie,
yes you are right, but I assumed if people see a cloud of condensed
fog over their LN2 bath they should remove that by
a) filling up the bowl completely e.g. some LN2 drips out of the bowl
b) blow the fog away before you dip
True; this has been demonstrated quite rigorously:
