Hi Peter,
You can specify in DM the mean density of the protein region in the SOLC
keyword. However I am not sure this will affect the density distribution
used for histogram matching.
All the best,
Adam.
And to add another small item: the SOLOMON interface in SHARP allows
adjustment of the mean density depending on protein, DNA or RNA
content. See
http://www.globalphasing.com/sharp/manual/denmod2.html#MeanDensity
Cheers
Clemens
On Wed, Aug 19, 2009 at 09:26:34AM +0100, Gerard Bricogne wrote:
Dear Ed,
The question of dealing appropriately with density modification in the
presence of "heavy atoms" has been discussed in the paper on SHARP 2.0 (see
Acta D59, 2023-2030, published in 2003) and the solution described in that
paper has been available in all versions of SHARP/autoSHARP si
Peter Grey wrote:
Hi everyone,
I am trying to use density modification at rather low resolution (4-5A )
for an RNA structure. My first time ever with RNA.
I usually use Histogram matching as part of the density modification
scheme in DM. But this method is based on density distribution of
pro
Hi Peter,
Histogram matching works well for RNA structure, but be aware that
density modification in general can be very tricky at low resolution.
If by any means you can exploit averaging, e.g. multicrystal averaging
of non-isomorphous crystals, it will probably be very helpful. It will
Hi everyone,
I am trying to use density modification at rather low resolution (4-5A ) for
an RNA structure. My first time ever with RNA.
I usually use Histogram matching as part of the density modification scheme
in DM. But this method is based on density distribution of protein maps I
think.
Is h
