Ion exchange chromatography, this is the published method.
Acoot
From: Xinghua Qin
To: CCP4BB@JISCMAIL.AC.UK
Sent: Monday, 4 June 2012 2:51 PM
Subject: [ccp4bb] How to know if ADP exists in the ATP-binding site of
bacterial expressed proteins
Deer CCP4ers,
How to know if ADP exists in
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of
Xinghua Qin
Sent: Monday, June 04, 2012 7:52 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] How to know if ADP exists in the ATP-binding site of
bacterial expressed proteins
Deer CCP4ers,
How to know
Deer CCP4ers,
How to know if ADP exists in the ATP-binding site of bacterial expressed
proteins? Check the UV spectrum at 280 nm is one way, but there is no Trp in my
protein. Is there any other conveniet way to find out?
Thanks in advance
Best wishes
Xinghua Qin
--
Xinghua Qin
State Key
