Anindito,
First off, I have never tried this, but here are a few initial thoughts on how
I might approach this. But first a question, how tight is the dimer form? if
you mixed dimers with tags and dimers without tags, would you get any exchange?
If so, you might be able to use this to your
Nicholas is right that it depends a lot on the dimer you are working
with. I worked on a dimeric tRNA synthetase and was able to make
heterodimers in a couple of ways. The most effective was to make a
bicistronic version of the ORFs each with their own S-D. Each had a tag
for tandem affinity
Hello Anindito,
While Nick and Paul have thrown you some great ideas around the TAP-tag
theme (although GST may not be ideal as it dimerises itself), I feel that
I've read your initial e-mail differently from them. Maybe you could
clarify?
It sounds like you have a specific tag of ~5kDa in
Dear All,
I need to insert a tag protein of ~5 kDa in one of the molecules of a dimer
protein (size is ~100kDa). To be more precise - The tag need to get itself
attached only on one of the dimer molecules.
My expertise are not in Cell biology and therefore any suggestion in this
regard
