>
> **
>
>
>
> *De: *bogba...@yahoo.co.uk
> *Enviado:*sexta-feira, 10 de julho de 2020 00:30
> *Para: *Rafael Marques
> *Cc:*CCP4BB@JISCMAIL.AC.UK
> *Assunto: *Re: [ccp4bb] RES: [ccp4bb] Looking for suggestions with
&g
[Rafael Marques](mailto:rafael_mmsi...@hotmail.com)
> Cc:CCP4BB@JISCMAIL.AC.UK
> Assunto: Re: [ccp4bb] RES: [ccp4bb] Looking for suggestions with protein
> expression
>
> Re: "In our lab we generally use BL21 for plasmid replication."
>
> I am interested because on t
.@yahoo.co.uk>
Enviado:sexta-feira, 10 de julho de 2020 00:30
Para: Rafael Marques<mailto:rafael_mmsi...@hotmail.com>
Cc:CCP4BB@JISCMAIL.AC.UK<mailto:CCP4BB@JISCMAIL.AC.UK>
Assunto: Re: [ccp4bb] RES: [ccp4bb] Looking for suggestions with protein
expression
Re: "In our lab
e São Paulo
>
>
>
> Bacharel em Ciências Biológicas
>
> Universidade Federal de São Carlos
>
>
>
> phone: +55 16 99766-0021
>
>
>
> * "A sorte acompanha uma mente bem treinada"*
>
> **
>
>
>
te bem treinada"
De: Lau Kelvin<mailto:kelvin@epfl.ch>
Enviado:quarta-feira, 8 de julho de 2020 16:22
Para: CCP4BB@JISCMAIL.AC.UK<mailto:CCP4BB@JISCMAIL.AC.UK>
Assunto: Re: [ccp4bb] Looking for suggestions with protein expression
Hello Umar,
I would not pin down your
Re: "In our lab we generally use BL21 for plasmid replication."I am interested because on the occasions that I prepared plasmids in BL21 (usually by mistake), they were never any good for cloning or sequencing. I always had to transform them back into a cloning strain and do another plasmid prep!
inada"
De: Lau Kelvin<mailto:kelvin@epfl.ch>
Enviado:quarta-feira, 8 de julho de 2020 16:22
Para: CCP4BB@JISCMAIL.AC.UK<mailto:CCP4BB@JISCMAIL.AC.UK>
Assunto: Re: [ccp4bb] Looking for suggestions with protein expression
Hello Umar,
I w
Hello Umar,
I would not pin down your difficulties solely due to an Fe-S proteins. I have
produced some with no fusion partners and they work wonderfully. They were
expressed in an aerobic environment and then reduced in an anaerobic one before
usage in reactions.
1) On the Fe-S side, there ar
the protein.
Good luck!
Ingrid
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Umar
Farook
Sent: Saturday, June 27, 2020 6:09 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Looking for suggestions with protein expression
Glutathione beads for GST tagged
d on behalf of Umar Farook
Sent: Saturday, June 27, 2020 6:08:51 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Looking for suggestions with protein expression
Glutathione beads for GST tagged protein.
Umar
On Sat, 27 Jun 2020, 6:56 pm John Newitt,
mailto:newit...@gmail.com>> wrote
Glutathione beads for GST tagged protein.
Umar
On Sat, 27 Jun 2020, 6:56 pm John Newitt, wrote:
> On Jun 27, 2020, at 3:15 AM, Umar Farook wrote:
> >
> >
> > Dear All,
> >
> > Sorry for an offtopic question, your suggestions are highly appreciated.
> >
> > We have been working on iron sulfur
On Jun 27, 2020, at 3:15 AM, Umar Farook wrote:
>
>
> Dear All,
>
> Sorry for an offtopic question, your suggestions are highly appreciated.
>
> We have been working on iron sulfur cluster binding protein, which is usually
> expressed as a nice soluble protein expressed in BL21 cells but agg
Hi
It is hard to give specific advice without knowing details about your
protein. There are multiple reasons that may be responsible for it's
behavior (fundamentally the simplest reason is that proteins by and large
are not designed by Nature to be purified and handled) so the more you
share with
Dear All,
Sorry for an offtopic question, your suggestions are highly appreciated.
We have been working on iron sulfur cluster binding protein, which is
usually expressed as a nice soluble protein expressed in BL21 cells but
aggregated in the affinity column itself and unable to recover from it.
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