dollins,
I do have an excellent reference for you to read
J Chromatogr B Analyt Technol Biomed Life Sci. 2002 Mar 25;769(1):133-44.
Strategies for the purification and on-column cleavage of
glutathione-S-transferase fusion target proteins.Dian C, Eshaghi S,
Urbig T, McSweeney S, Heijbel A, Salbert
-Original Message-
From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On Behalf Of Eric
Dollins
Sent: Thursday, February 28, 2008 2:26 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Off topic: General rule for maximum flow rate for affinity
column?
Dear protein purifiers,
Off topic
the protein.
Good luck. Happy purifying!
Artem
-Original Message-
From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On Behalf Of Eric
Dollins
Sent: Thursday, February 28, 2008 5:26 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Off topic: General rule for maximum flow rate for affinity
column
Eric,
I second Chun's reply. As the Volumetric flowrate (ml/min) is equal
to the linear flowrate times the area of the column, and the linear
flowrate is fixed for a particular resin (Sigma is terrible at providing
this value for their resins) you basically just need to increase the
