014 2:36 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] P31 or P32
But are you sure it is twinned and not really P312?
Ltest etc is a pretty reliable indicator but the results can be biased by
non-crystallographic translation, etc
And do the HA sites obey the twinning symmetry?
On 16 Dec
ISCMAIL.AC.UK] On Behalf Of
> RHYS GRINTER
> Sent: Tuesday, December 16, 2014 10:37 AM
> To: CCP4BB@JISCMAIL.AC.UK
> Subject: Re: [ccp4bb] P31 or P32
>
> Hi Eleanor,
>
> The data is perfectly twinned, with the original auto processing assigning
> a point group of P312, but I r
] On Behalf Of RHYS
GRINTER
Sent: Tuesday, December 16, 2014 10:37 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] P31 or P32
Hi Eleanor,
The data is perfectly twinned, with the original auto processing assigning a
point group of P312, but I reprocessed it in P3 and it seems to be behaving
From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] On Behalf Of Eleanor Dodson
[eleanor.dod...@york.ac.uk]
Sent: 16 December 2014 15:13
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] P31 or P32
Sorry - of course it can.. Sorry.
Only if the twinning is perfect then you
Sorry - of course it can.. Sorry.
Only if the twinning is perfect then you apparently get a higher
symmetry..
Eleanor
On 16 December 2014 at 14:26, Tim Gruene wrote:
>
> Dear Rhys,
>
> I would try to place idealised secondary structure elements with coot
> into the density - at this resolution t
Dear Rhys,
I would try to place idealised secondary structure elements with coot
into the density - at this resolution they probably fit both hands, but
you may see a difference when you do e.g. rigid body refinement.
Best,
Tim
On 12/16/2014 10:39 AM, RHYS GRINTER wrote:
> Hi All,
>
> This will
What do the twinning tests show? Those space groups can be suspicious, I
believe.
JPK
-Original Message-
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of RHYS
GRINTER
Sent: Tuesday, December 16, 2014 4:40 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] P31 or P32
If your Pt position is "centric" then either hand will give a reasonable
looking solution but one will generate LH helices and the other right.
Model building can break that ambiguity - SHELXE and Buccaneer both will
impose correct peptide geometry.
But at such low resolution they may struggle.. b
Hi All,
This will no doubt show something of my ignorance with experimental phasing,
however I'm currently working on solving a 3.8A SAD dataset with Pt anomalous
signal. Both Shelx and Xtriage see reasonable anomalous signal to about 7A and
seem to get statistics suggesting a solution when I
